Method for cleaning reaction vessels in place

• US 20050170512A1
• Filed: 10/28/2004
• Published: 08/04/2005
• Est. Priority Date: 10/28/2003
• Status: Abandoned Application

• Current Assignee: Brooks Automation Ltd. (United Kingdom) (Azenta, Inc.)
• Original Assignee: Brooks Automation Ltd. (United Kingdom) (Azenta, Inc.)
• Inventors: Haines, Dina, Hembree, Sarah, Rubin, Sean, Gordon, Steven J.
• Application Number:
  US10/975,824
• Publication Number:
  US 20050170512A1
• Time in Patent Office: Days
• US Class Current: 436/43
• CPC Class Codes: A61L 2/18 Liquid substances or solutions comprising solids or dissolved gases
• CPC Class Codes: B01L 13/02 for receptacle or instruments
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/1844 using fans
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B08B 9/08 Cleaning containers, e.g. tanks
• CPC Class Codes: B08B 9/22 the apparatus cleaning by soaking alone
• CPC Class Codes: G01N 2035/0437 Cleaning cuvettes or reaction vessels
• CPC Class Codes: Y10T 436/11 Automated chemical analysis

Abstract

A method and system for reusing reaction vessels in a biotechnical processing apparatus. The system has a frame, an array of reaction vessels, an automatic actuator, and a decontamination system. The frame has at least one processing station. The array of reaction vessels is mounted to the frame for effecting biotechnical processing at the precessing station. The automatic actuator is connected to the frame and the reaction vessels for dispersion of solution in and out of the reaction vessels. The decontamination system is constructed in the frame for being accessed by the actuator to decontaminate the reaction vessels. The decontamination system includes a wash section, a heating section, a supply of wash fluid, a supply of acid solution and a supply of base solution. The actuator is programmed to access the decontamination section to subject the access the decontamination section, a bas solution and a wash reagent for decontamination the reaction vessels.

Claims

1. A method of reusing reaction vessels in a biotechnical application comprising

providing a biotechnical processing apparatus with at least one processing station for performing a biotechnical process., and an automatic actuator for charging reagents into a reaction vessel to perform a biotechnical process in the reaction vessel at the at least one processing station, the apparatus having an integral cleaning system for cleaning the reaction vessels when contaminated;

with the actuator, introducing an acid solution from the integral cleaning system into the reaction vessel;

with the integral cleaning system, heating the acid solution;

with the actuator, eliminating the acid solution from the reaction vessel;

with the actuator, introducing a base solution from the integral cleaning system into the reaction vessel;

with the actuator, eliminating the base solution from the reaction vessel; and

with the integral cleaning system, flushing the reaction vessel with a wash fluid.

2. The method of reusing reaction vessels, according to claim 1, further comprising agitating the acid filled reaction vessel with the integral cleaning system to cover a predetermined contaminated portion of the reaction vessels with the acid solution.

3. The method of reusing reaction vessels, according to claim 1, wherein introduction of the acid solution comprises operating a plunger with the actuator to aspirate the acid.

4. The method of reusing reaction vessels, according to claim 3, wherein agitation is accomplished by cycling the plunger.

5. The method of reusing reaction vessels according to claim 1, further comprising providing the actuator with a robot for moving the reaction vessel relative to the at least one processing station, moving, with the robot, the reaction vessel to a wash station of the apparatus and dispensing process residues from the reaction vessel;

causing the robot to move the contaminated reaction vessel to an acid station of the cleaning system for aspirating the acid solution into the reaction vessels;

causing the robot to move the acid filled reaction vessels to a thermal station of the cleaning system for heating the acid solution in the reaction vessel;

causing the robot to move the heated reaction vessel to wash station of the apparatus, for dispensing the acid solution from the reaction vessels;

causing the robot to move the reaction vessel to a base station of the cleaning system for aspirating the base solution into the reaction vessel; and

causing the robot to move the reaction vessel to the wash station for dispensing the base solution from the reaction vessel.

6. The method of reusing reaction vessels, according, to claim 2, wherein the reaction vessel containing the base solution is agitated to cover the predetermined contaminated portion of the reaction vessel with the base solution.

7. The method of reusing reaction vessels, according to claim 6, wherein the agitation step is accomplished by cycling a plunger with the actuator.

8. The method of reusing reaction vessels, according to claim 1, wherein the acid is heated to a temperature in the range of 28 to 200 degrees Celsius.

9. The method of reusing reaction vessels, according to claim 1, wherein the acid is nitric acid in a 0.001 to 15 molar solution.

10. The method of reusing reaction vessels according to claim 1, wherein the base is Potassium Hydroxide, in a 0.001 and 5 normal solution.

11. The method of reusing reaction vessels, according to claim 1, wherein the reaction vessel is made of glass.

12. The method of reusing reaction vessels, according to claim 1, wherein the reaction vessel interior is coated with a strippable coating before use in the biotechnical process and wherein the strippable coating is removed during decontamination.

13. The method of reusing reaction vessels, according to claim 12, wherein the strippable coating is a silinization coating.

14. The method of reusing reaction vessels, according to claim 1, wherein the biotechnical process is a polymerase chain reaction.

32. The method of decontaminating reaction vessels, according to claim 4, wherein the agitation means comprises a source of ultrasound vibrations for application to the reaction vessels to insure coverage of the reaction vessels by the acid and base solutions.

15. A system for performing biotechnical processing, comprising:

a frame having at least one processing station constructed therein;

an array of reaction vessels mounted to the frame for effecting biotechnical processing at the at least one processing station;

an automatic actuator connected to the frame and said reaction vessels for dispersion of solution into and out of the reaction vessels;

a decontamination system constructed in the frame for being accessed by the actuator to decontaminate the reaction vessels, the decontamination section further comprising;

a wash section connected to the frame to receive and remove fluids from the reaction vessels;

a heating section connected to the frame to heat the reaction vessels;

a supply of wash fluid, a supply of acid solution and a supply of base solution connected to the frame for application to the reaction vessels; and

wherein said actuator is programmed to access said decontamination section to subject the reaction vessels to;

an acid solution, heat, a base solution, and a wash reagent for decontaminating the reaction vessels.

16. The system for performing biotechnical processing, according to claim 15, wherein the decontamination section further comprises:

an acid bath station constructed in the frame and connected to the supply of acid solution; and

a base bath station constructed in the frame and connected to the supply of base solution.

17. The system for performing biotechnical processing, according to claim 15, wherein the wash station comprises:

a retaining block having apertures constructed therein, the apertures being formed to accommodate the passage of the reaction vessels; and

a wash chamber accessible to the reaction vessels through the apertures and connected to flush the acid solution, base solution, and wash fluid applied to the reaction vessels.

18. The system for performing biotechnical processing, according to claim 15, wherein the heating station comprises.

a heating chamber to accommodate the array of reaction vessels;

and

a source of heated air connected to circulate heated air into the heating chamber.

19. The system for performing biotechnical processing, according to claim 18, further comprising a process controller with programming arranged so that upon execution, the programming causes said system to perform a decontamination process comprising:

causing the actuator to dispense residue from said array of reaction vessels into the wash section;

causing the actuator to access supply of acid solution to fill said reaction vessels with the acid solution;

causing the actuator to interface with said heating section to heat the acid solution filled reaction vessels;

causing the actuator to flush the acid solution from the reaction vessels into the wash section;

causing the actuator to access the supply of base solution to fill the reaction vessels with base solution;

causing the actuator to flush the base solution from the reaction vessels into the wash section; and

causing the actuator to access the supply of wash fluid to flush the reaction vessels.

20. The system for performing biotechnical processing, according to claim 19, wherein the heated acid solution in the reaction vessels is agitated by at least one of the actuator or the decontamination system to cover a contaminated portion of the reaction vessel with the acid solution.

21. The system for performing biotechnical processing, according to claim 19, wherein the base solution in the reaction vessels is agitated by at least one of the actuator or the decontamination system to cover a contaminated portion of the reaction vessel with the base solution.

22. The system for performing biotechnical processing, according to claim 19, wherein the acid is heated in the heat station to a temperature in the range of 28 to 200 degrees Celsius.

23. The system for performing biotechnical processing, according to claim 19, wherein the acid is nitric acid in a 0.001 to 15 molar solution.

24. The system for performing biotechnical processing, according to claim 19, wherein the base is Potassium Hydroxide, in a 0.001 and 5 normal solution.

25. The system for performing biotechnical processing, according to claim 19, wherein, the reaction vessels are made of glass.

26. The system for performing biotechnical processing, according to claim 19, wherein an interior of the reaction vessels is coated with a strippable coating and wherein the strippable coating is removed during the method of decontamination.

27. The system for performing biotechnical processing, according to claim 26, wherein the strippable coating is a silinization coating.

28. The system for performing biotechnical processing, according to claim 19, wherein the actuator comprises a robot movably connected to the frame and capable of holding the reaction vessels to transport the vessels between at least the wash section and heating section, wherein the robot is capable of applying a vacuum to the array of reaction vessels too aspirate the acid solution and the base solution into the reaction vessels.

29. The system for performing biotechnical processing, according to claim 15, wherein an agitator is provided to agitate the reaction vessels after they are filled with the acid solution and again after they are filled with the base solution.

30. The system for performing biotechnical processing according to claim 15, further comprising a controller communicably connected to the actuator and decontamination system, wherein the controller comprises a control algorithm for repetitive cycling of at least one of the actuator or the decontamination system to insure coverage of the reaction vessels by the acid solution and the base solution.

31. The system for performing biotechnical processing, according to claim 15, wherein the biotechnical processing comprises a polymerase chain reaction.