Biosensor
First Claim
1. A biosensor, comprising:
- an electrode system including at least one pair of electrodes;
at least one insulating base plate for supporting the electrode system;
a first reaction layer provided at least on a working electrode of the electrode system, including an organic compound having a functional group capable of bonding or being adsorbed to the electrode and a hydrophobic hydrocarbon group;
a second reaction layer provided on the first reaction layer, including an amphiphilic lipid capable of bonding or being adsorbed to a hydrophobic portion of the first reaction layer; and
a reagent system carried in a two-component membrane composed of the first and second reaction layers, including at least membrane-binding type pyrroquinoline quinone-dependent glucose dehydrogenase and an electron mediator.
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Accused Products
Abstract
The present invention relates to a biosensor which comprises an electrode system including at least one pair of electrodes, at least one insulating base plate for supporting the electrode system, a first reaction layer provided at least on a working electrode of the electrode system, including an organic compound having a functional group capable of bonding or being adsorbed to an electrode and a hydrophobic hydrocarbon group, a second reaction layer provided on the first reaction layer, including an amphiphilic lipid capable of bonding or being adsorbed to a hydrophobic portion of the first reaction layer, and a reagent system carried in a two-component membrane composed of the first and second reaction layers, including at least membrane-binding type pyrroquinoline quinone-dependent glucose dehydrogenase and an electron mediator.
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Citations
24 Claims
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1. A biosensor, comprising:
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an electrode system including at least one pair of electrodes;
at least one insulating base plate for supporting the electrode system;
a first reaction layer provided at least on a working electrode of the electrode system, including an organic compound having a functional group capable of bonding or being adsorbed to the electrode and a hydrophobic hydrocarbon group;
a second reaction layer provided on the first reaction layer, including an amphiphilic lipid capable of bonding or being adsorbed to a hydrophobic portion of the first reaction layer; and
a reagent system carried in a two-component membrane composed of the first and second reaction layers, including at least membrane-binding type pyrroquinoline quinone-dependent glucose dehydrogenase and an electron mediator. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method for fabricating a biosensor, comprising the steps of:
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forming an electrode system including at least one pair of electrodes on an insulating base plate;
providing a first reaction layer at least on a working electrode of the electrode system, wherein a solution containing an organic compound having a functional group capable of bonding or being adsorbed to the electrode and a hydrophobic hydrocarbon group is made contact with the working electrode; and
providing a second reaction layer on the first reaction layer, wherein a solution containing an amphiphilic lipid is made contact with the first reaction layer, wherein at least one of the solution containing the organic compound and the solution containing the amphiphilic lipid contains an electron mediator, and the solution containing the amphiphilic lipid further contains membrane-binding type pyrroquinoline quinone-dependent glucose dehydrogenase. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification