Self-hybridizing multiple target nucleic acid probes and methods of use
First Claim
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1. A nucleic acid probe comprising:
- (a) a first probe region specific for a first target sequence; and
(b) a second probe region specific for a second target sequence, wherein;
(i) the first probe region and the second probe region are non-contiguous, the first target sequence and the second target sequence are non-contiguous, or both the first probe region and the second probe region and the first target sequence and the second target sequence are non-contiguous; and
(ii) the nucleic acid probe comprises self-complementary sequences.
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Abstract
The present invention provides probes for target nucleic acid sequences and methods for using the probes to detect the presence or absence of a desired target nucleic acid sequence. The present probes contain two or more probing regions. The probes also contain multiple self-complementary sequences that hybridize to one another in the absence of one or more of the target sequences. The probes can also optionally contain one or more arm sequences, spacer sequences or labels as desired.
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Citations
45 Claims
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1. A nucleic acid probe comprising:
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(a) a first probe region specific for a first target sequence; and
(b) a second probe region specific for a second target sequence, wherein;
(i) the first probe region and the second probe region are non-contiguous, the first target sequence and the second target sequence are non-contiguous, or both the first probe region and the second probe region and the first target sequence and the second target sequence are non-contiguous; and
(ii) the nucleic acid probe comprises self-complementary sequences. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45)
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Specification