Methods and devices for retrieving a subject's physiological history from a biological sample analysis

• US 20050176089A1
• Filed: 02/08/2004
• Published: 08/11/2005
• Est. Priority Date: 12/04/2003
• Status: Abandoned Application

• Current Assignee: Michael Scott Ehrlich
• Original Assignee: Michael Scott Ehrlich
• Inventors: Ehrlich, Michael Scott
• Application Number:
  US10/774,681
• Publication Number:
  US 20050176089A1
• Time in Patent Office: Days
• US Class Current: 435/40.500
• CPC Class Codes: G01N 2400/10 Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
• CPC Class Codes: G01N 33/66 involving blood sugars, e.g. galactose

Abstract

Methods and devices for retrieving a subject'"'"'s physiological history from a single sample or measurement are disclosed. Methods and devices for obtaining a blood glucose history from a single blood sample are disclosed.

Claims

1. A method of obtaining a subject'"'"'s physiological history from a sample analysis, the method comprising the steps of:

(a) measuring an amount of a cellular modification for each of a plurality of cells from a biological sample;

(b) sorting the cell measurements from step (a) as a function of the amount of modification; and

,

(c) determining a physiological history for the subject by analyzing the ordered cell measurements.

2. The method of claim 1, wherein the physiological history is indicative of a disease or disorder in the subject.

3. The method of claim 2, wherein the disease or disorder is diabetes.

4. The method of claim 3, wherein the diabetes is Type I diabetes.

5. The method of claim 3, wherein the diabetes is Type II diabetes.

6-18. -18. (canceled)

19. A method of obtaining a cell distribution profile of a patient, the method comprising the steps of:

(a) obtaining a biological sample comprising intact cells; and

(b) determining a cell distribution profile for a plurality of the intact cells using a procedure that sorts the intact cells based on an amount of a physiological variable in each of the sorted cells.

20-24. -24. (canceled)

25. A method for recovering the blood glucose level history of a patient diagnosed with diabetes, the method comprising the steps of:

(a) obtaining a blood sample comprising intact cells

(b) obtaining a cell distribution profile by sorting a plurality of the intact cells based on the amount of glycated hemoglobin in each cell; and

,

(c) determining the patient'"'"'s glucose level history based on the cell distribution profile.

26. The method of claim 25, wherein the patient'"'"'s blood glucose level history is obtained by comparing the patient'"'"'s cell distribution profile to one or more reference profiles.

27. The method of claim 25, wherein the patient'"'"'s blood glucose level history is obtained using an algorithm.

28. The method of claim 27, wherein the algorithm corrects for non-linearity in the rate of hemoglobin glycation.

29. The method of claim 27, wherein the algorithm uses patient specific input data.

30. The method of claim 29, wherein the patient specific input data is selected from the group consisting of the patient'"'"'s age, gender, and weight.

31. The method of claim 27, wherein the algorithm uses a value for the average life span of a red blood cell.

32. The method of claim 25, wherein the intact cells are sorted using a procedure selected from the group consisting of chromatography, electrophoresis, mass spectrometry, and cell sorting.

33. The method of claim 25, comprising the additional step of determining whether the patient has Type I, Type II, or gestational diabetes.

34. The method of claim 25, comprising the additional step of determining a treatment regimen for the patient.

35. The method of claim 25, comprising the step of obtaining a second blood sample at a second time and performing steps (b) and (c) on the second blood sample.

36. The method of claim 25, wherein the glycosylated hemoglobin is labeled.

37. A chromatography device for separating blood cells based on levels of glycosylated hemoglobin, the device comprising:

(a) a high pressure column comprising a sample chamber and a tapered exit hole;

(b) a dynamic light scattering detector connected to the exit hole; and

,

(c) a gas powered injector connected to the sample chamber.

38. An electrophoretic device for separating blood cells based on levels of glycosylated hemoglobin, the device comprising:

(a) a liquid flow column with a sample entry point and a plurality of collection points along the length of the column; and

,

(b) an electromagnetic field generator associated with the column, wherein the electromagnetic field is normal to the direction of the liquid flow.