Process for producing a-hydroxy acid ammonium salt
First Claim
1. A method for producing α
- -hydroxy acid ammonium salt represented by RCH(OH)COO−
NH4+ of general formula (II) characterized in that, when converting an α
-hydroxynitrile represented by RCH(OH)CN of general formula (I) (wherein, R represents a hydrogen atom, a C1 to C6 alkyl group which may be substituted, a C2 to C6alkenyl group which may be substituted, a C1 to C6 alkoxyl group which may be substituted, an aryl group which may be substituted, an aryloxy group which may be substituted or a heterocyclic group which may be substituted) to an α
-hydroxy acid ammonium salt represented by RCH(OH)COO−
NH4+ of general formula (II) (wherein R is the same as previously defined), a microbial catalyst originating in a microbial strain is used that is capable of maintaining the average production rate of the α
-hydroxy acid ammonium salt represented by general formula (II) at at least 100 μ
mol/min per g of dry microbial cell weight for 14 days or more without adding fresh microbial catalyst.
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Abstract
The present invention provides a method for producing. a-hydroxy acid ammonium salt that uses a specific microbial strain capable of accumulating α-hydroxy acid ammonium salt at a high concentration while also maintaining an industrially satisfactory production rate for a long period of time. More specifically, the present invention uses a microbial catalyst originating in a microbial strain which, during conversion of α-hydroxynitrile to α-hydroxy acid ammonium salt, is capable of maintaining the average production rate of α-hydroxy acid ammonium salt at at least 100 μmol/min per g of dry microbial cell weight for 14 days or more without adding fresh microbial catalyst, and is capable of accumulating the α-hydroxy acid ammonium salt at 20 to 60% by weight. An example of this microbial strain is Arthrobacter sp. strain NSSC204.
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Citations
13 Claims
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1. A method for producing α
- -hydroxy acid ammonium salt represented by RCH(OH)COO−
NH4+ of general formula (II) characterized in that, when converting an α
-hydroxynitrile represented by RCH(OH)CN of general formula (I) (wherein, R represents a hydrogen atom, a C1 to C6 alkyl group which may be substituted, a C2 to C6alkenyl group which may be substituted, a C1 to C6 alkoxyl group which may be substituted, an aryl group which may be substituted, an aryloxy group which may be substituted or a heterocyclic group which may be substituted) to an α
-hydroxy acid ammonium salt represented by RCH(OH)COO−
NH4+ of general formula (II) (wherein R is the same as previously defined), a microbial catalyst originating in a microbial strain is used that is capable of maintaining the average production rate of the α
-hydroxy acid ammonium salt represented by general formula (II) at at least 100 μ
mol/min per g of dry microbial cell weight for 14 days or more without adding fresh microbial catalyst. - View Dependent Claims (2, 3, 4, 5, 6, 7)
- -hydroxy acid ammonium salt represented by RCH(OH)COO−
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8. A method for producing α
- -hydroxy acid ammonium salt represented by general formula (II), wherein the α
-hydroxynitrile is lactonitrile, acetone cyanohydrin, mandelonitrile or 2-hydroxy-4-methylthiobutyronitrile.
- -hydroxy acid ammonium salt represented by general formula (II), wherein the α
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9. A microbial strain characterized in that, when converting an α
- -hydroxynitrile represented by RCH(OH)CN of general formula (I) (wherein, R represents a hydrogen atom, a C1 to C6 alkyl group which may be substituted, a C2 to C6 alkenyl group which may be substituted, a C1 to C6 alkoxyl group which may be substituted, an aryl group which may be substituted, an aryloxy group which may be substituted or a heterocyclic group which may be substituted) to an α
-hydroxy acid ammonium salt represented by RCH(OH)COO−
NH4+ of general formula (II) (wherein R is the same as previously defined), is capable of maintaining the average production rate of the α
-hydroxy acid ammonium salt represented by general formula (II) at at least 100 μ
mol/min per g of dry microbial cell weight for 14 days or more without adding fresh microbial catalyst. - View Dependent Claims (10, 11, 12, 13)
- -hydroxynitrile represented by RCH(OH)CN of general formula (I) (wherein, R represents a hydrogen atom, a C1 to C6 alkyl group which may be substituted, a C2 to C6 alkenyl group which may be substituted, a C1 to C6 alkoxyl group which may be substituted, an aryl group which may be substituted, an aryloxy group which may be substituted or a heterocyclic group which may be substituted) to an α
Specification