Implantable biosensor and methods of use thereof
First Claim
1. A sensor, comprising a) an oxygen transport region comprising a first reversible oxygen binding protein, an oxygen permeable first surface in communication with an external environment, and an oxygen permeable second surface which is impermeable to target analyte;
- b) a target analyte reaction region in communication with the oxygen transport region at the oxygen permeable second surface, wherein the target analyte reaction region comprises a target analyte oxidase enzyme, and a target analyte-permeable surface; and
c) a sensing region comprising at least one detector probe in communication with the target analyte reaction region.
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Abstract
Provided herein is a stabilized oxygen transport matrix that includes a reversible oxygen binding protein, such as hemoglobin, immobilized throughout the stabilized oxygen transport matrix. The stabilized oxygen transport matrix is used to transport oxygen and can be used as an oxygen transport region and a reaction region of an analyte sensor, such as an implantable glucose sensor. The reversible binding protein can also function as an oxygen probe within the analyte sensor.
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Citations
55 Claims
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1. A sensor, comprising
a) an oxygen transport region comprising a first reversible oxygen binding protein, an oxygen permeable first surface in communication with an external environment, and an oxygen permeable second surface which is impermeable to target analyte; -
b) a target analyte reaction region in communication with the oxygen transport region at the oxygen permeable second surface, wherein the target analyte reaction region comprises a target analyte oxidase enzyme, and a target analyte-permeable surface; and
c) a sensing region comprising at least one detector probe in communication with the target analyte reaction region. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A method for measuring a concentration of an analyte, comprising
transporting oxygen from an external environment through an oxygen transport region within a sensor to an analyte reaction region of the sensor using a first reversible oxygen binding protein; -
reacting a portion of the transported oxygen with the analyte enzymatically in the analyte reaction region to form a product; and
measuring oxygen or a product of the reaction of oxygen and the analyte in a sensing region comprising the analyte reaction region or a sensing region in contact with the analyte reaction region, thereby measuring the concentration of the analyte. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48)
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- 49. A stabilized oxygen transport matrix, comprising a reversible oxygen binding protein immobilized throughout the stabilized oxygen transport matrix.
- 52. A method for transporting oxygen from a first area having a relatively higher oxygen concentration to a second area having a relatively lower oxygen concentration, comprising contacting oxygen from the first area with a first surface of a stabilized oxygen transport matrix that includes a reversible oxygen binding protein immobilized throughout the stabilized oxygen transport matrix, and transporting oxygen away from the first surface of the stabilized oxygen transport matrix to a second surface of the oxygen transport matrix, wherein the second surface of the oxygen transport matrix contacts the second area.
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55. The method of claim D1, wherein the transporting is carried out by an oxygen binding protein immobilized in the matrix at a concentration sufficient to measurably increase the flux of oxygen across the second surface of the oxygen transport matrix as compared to that achieved by simple diffusion.
Specification