Total internal reflection fluorescence microscope
First Claim
1. A total internal reflection fluorescence microscope comprising:
- a light source which generates laser light having a plurality of wavelengths;
a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination;
a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination;
an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen; and
a controller which controls the incident angle adjuster such that the amount of the permeation depth of the evanescent light is the same when the wavelength of the laser light from the laser light source is switched.
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Accused Products
Abstract
A total internal reflection fluorescence microscope includes a light source which generates laser light having a plurality of wavelengths, a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination, a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination, an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen, and a controller which controls the incident angle adjuster such that the amount of the permeation depth of the evanescent light is the same when the wavelength of the laser light from the laser light source is switched.
25 Citations
47 Claims
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1. A total internal reflection fluorescence microscope comprising:
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a light source which generates laser light having a plurality of wavelengths;
a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination;
a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination;
an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen; and
a controller which controls the incident angle adjuster such that the amount of the permeation depth of the evanescent light is the same when the wavelength of the laser light from the laser light source is switched. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A total internal reflection fluorescence microscope comprising:
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a light source which generates laser light having a plurality of wavelengths;
a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination;
a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination;
an incident angle adjuster which adjusts the incident angle of the laser light irradiated on the specimen; and
a controller which controls the incident angle adjuster such that the permeation depth of the evanescent light is set to a predetermined amount when the wavelength of the laser light from the laser light source is switched. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A total internal reflection fluorescence microscope comprising:
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a light source which generates laser light having a plurality of wavelengths;
a varying unit which variably controls the wavelength of the laser light and output intensity;
a focal optical system which irradiates the laser light from the light source on a specimen at a predetermined incident angle via an objective lens and which generates evanescent illumination; and
a fluorescence observation unit which observes fluorescence generated from the specimen due to the evanescent illumination. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
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41. A total internal reflection fluorescence microscope comprising:
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a light source;
an incident angle controller which controls the incident angle of the illumination light from the light source which is irradiated on the specimen;
a permeation depth calculator which calculates the depth of permeation of the specimen for total internal reflection illumination from a control value of the incident angle controller; and
a display unit which displays permeation depth calculated by the permeation depth calculator. - View Dependent Claims (42, 43, 45)
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44. A total internal reflection fluorescence microscope comprising:
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a light source;
an incident angle controller which controls the incident angle of the illumination light from the light source which is irradiated on the specimen;
an input unit which inputs a permeation depth of permeation of the specimen for total internal reflection illumination;
a condition incident angle calculator which calculates the conditions for calculating the incident angle to set the depth of permeation of the specimen for total internal reflection illumination to the input permeation depth; and
a display unit which displays the conditions incident angle obtained by the condition incident angle calculator. - View Dependent Claims (46, 47)
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Specification