Method of using fibrin-bound angiogenic factors to stimulate vascularization of transplant site of encapsulated cells
First Claim
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1. A method of stimulating vascularization at a transplant site in an animal comprising:
- providing a pharmaceutical composition comprising a fibrinogen, a fibrinolysis inhibitor, an angiogenic growth factor, one or more encapsulated cells, a thrombin and a divalent salt; and
administering the pharmaceutical composition at said transplant site to said animal.
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Abstract
The present invention relates to compositions and methods of treating a disease, such as diabetes, by implanting encapsulated biological material with a growth factor and conjugate into a patient in need of treatment. Several methods are presented to accomplish transplanting several different types of biological materials. This invention also provides methods of utilizing these encapsulated biological materials to treat different human and animal diseases or disorders by implanting them into several areas in the body including the subcutaneous site.
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Citations
46 Claims
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1. A method of stimulating vascularization at a transplant site in an animal comprising:
providing a pharmaceutical composition comprising a fibrinogen, a fibrinolysis inhibitor, an angiogenic growth factor, one or more encapsulated cells, a thrombin and a divalent salt; and
administering the pharmaceutical composition at said transplant site to said animal.- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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20. A method of preparing a pharmaceutical composition comprising:
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(a) preparing a solution comprising a fibrinogen, a fibrinolysis inhibitor, and an angiogenic growth factor to form a fibrinogen/fibrinolysis inhibitor/angiogenic growth factor solution;
(b) adding at least one encapsulated cell to the fibrinogen/fibrinolysis inhibitor/angiogenic growth factor solution; and
(c) adding a thrombin into the encapsulated cell/fibrinogen/fibrinolysis inhibitor/angiogenic growth factor solution of step (b) to produce a pharmaceutical composition comprising a thrombin, at least one encapsulated cell, fibrinogen, fibrinolysis inhibitor, and angiogenic growth factor. - View Dependent Claims (21, 22, 23, 24)
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25. A method of preparing a pharmaceutical composition comprising:
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(a) preparing a solution comprising a thrombin and an angiogenic growth factor to form a thrombin/angiogenic growth factor solution;
(b) adding at least one encapsulated cell to the thrombin/angiogenic growth factor solution; and
(c) adding a fibrinogen and a fibrinolysis inhibitor into the encapsulated cell/thrombin/angiogenic growth factor solution of step (b) to produce a pharmaceutical composition comprising a fibrinogen, a fibrinolysis inhibitor, at least one encapsulated cell, thrombin and an angiogenic growth factor. - View Dependent Claims (26, 27, 28, 29)
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30. A method of stimulating vascularization at a transplant site in an animal comprising:
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placing a first solution comprising fibrinogen, fibrinolysis inhibitor, angiogenic growth factor and at least one encapsulated cell into a first barrel of a syringe; and
placing a second solution comprising thrombin into a second barrel of a syringe;
and injecting the first and second solutions into an injection site on said animal. - View Dependent Claims (31, 32)
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33. A method of stimulating vascularization at a transplant site in an animal comprising:
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placing a first solution comprising thrombin, angiogenic growth factor and at least one encapsulated cell into a first barrel of a syringe;
placing a second solution comprising fibrinogen and fibrinolysis inhibitor into a second barrel of a syringe; and
injecting the first and second solutions into an injection site on said animal. - View Dependent Claims (34, 35)
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36. A pharmaceutical composition comprising:
- a fibrinogen, a fibrinolysis inhibitor, an angiogenic growth factor, one or more encapsulated cells, a thrombin and a divalent salt.
- View Dependent Claims (37, 38, 39, 40, 41, 42, 43, 44, 45, 46)
Specification