Methods of assaying physiological states
First Claim
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1. A method of generating and optionally using a responsive cellular profile corresponding to a biological state, the method comprising:
- a) providing a first sample from a first subject, wherein the first subject has a first biological state;
b) providing a first cell population;
c) contacting the first sample with the first cell population in vitro;
d) detecting a plurality of cellular constituents of the first cell population to generate a first responsive cellular profile, wherein the first responsive cellular profile corresponds to the first biological state. e) providing information that describes the first biological state;
f) optionally, creating a database linking the information that describes the first biological state to the first responsive cellular profile.
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Abstract
The present application provides, among other things, methods for monitoring biological states in a subject, as well as methods for monitoring the effect of interventions or therapies upon the biological states of a subject.
17 Citations
54 Claims
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1. A method of generating and optionally using a responsive cellular profile corresponding to a biological state, the method comprising:
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a) providing a first sample from a first subject, wherein the first subject has a first biological state;
b) providing a first cell population;
c) contacting the first sample with the first cell population in vitro;
d) detecting a plurality of cellular constituents of the first cell population to generate a first responsive cellular profile, wherein the first responsive cellular profile corresponds to the first biological state. e) providing information that describes the first biological state;
f) optionally, creating a database linking the information that describes the first biological state to the first responsive cellular profile. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 47, 48, 49, 50, 51, 52, 53, 54)
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18. A method of generating and optionally using a responsive cellular profile corresponding to a biological state comprising:
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a) providing a first tissue or cellular sample from a first subject, wherein the first subject has a first biological state;
b) detecting a plurality of cellular constituents of cells of the first cellular sample to generate a first responsive cellular profile, wherein the first responsive cellular profile corresponds to the first biological state. - View Dependent Claims (19, 20)
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21. A method of determining the presence, intensity, stage, or level, of a biological state of a subject, the method comprising:
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a) providing a first responsive cellular profile corresponding to the physiological state of the subject;
b) providing one or more additional responsive cellular profiles corresponding to one or more known related physiological states of the same or different subjects;
c) comparing the first responsive cellular profile to the one or more additional responsive profiles to identify similar cellular profiles, wherein the physiological state of the subject is similar to the known physiological state that corresponds to the similar cellular profiles. - View Dependent Claims (23, 24, 25)
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22. A method of determining the presence, intensity, stage, or level, of a biological state of a subject, the method comprising:
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a) providing a plurality of responsive cellular profiles corresponding to a plurality of known related biological states of one or more subjects;
b) analyzing the plurality of responsive cellular profiles corresponding to a plurality of known related biological states to generate an inferential set;
c) providing a responsive cellular profile corresponding to a subject having an unknown physiological state;
d) using the inferential set to predict the biological state of the subject having an unknown biological state. - View Dependent Claims (26, 27)
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28. A method of determining the presence, intensity, stage, or level, of one or more physiological states of a subject, said method comprising:
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a) providing an inferential set comprising, for each physiological state, a set of levels of a plurality of cellular constituents, wherein the variation in the levels of the cellular constituents is predictive of physiological state;
b) providing a responsive cellular profile for the subject;
c) extracting from the inferential set one or a combination of calculated predictive profiles for which similarity is greatest between the responsive cellular profile and the calculated predictive profiles, wherein each calculated predictive profile corresponds to a predicted level of a physiological state of the subject. - View Dependent Claims (29, 41, 42, 43, 44, 45)
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30. A method of determining a level of effect of one or more therapies upon a subject, said method comprising:
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a) providing an inferential set comprising, for each therapy, a set of levels of a plurality of cellular constituents, wherein the variation in the levels of the cellular constituents is predictive of the level of effect of a therapy;
b) providing a responsive cellular profile for the subject;
c) extracting from the inferential set one or a combination of calculated predictive profiles for which similarity is greatest between the responsive cellular profile and the calculated predictive profiles, wherein each calculated predictive profile corresponds to a predicted level of effect of a therapy on the subject. - View Dependent Claims (31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 46)
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Specification