Substrate preparation process
First Claim
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1. A method of forming an array of polymers on a surface of a substrate, comprising:
- providing a substrate having a first surface coated with functional groups protected with a photolabile protecting group, and a second surface having a layer disposed thereon, said layer including one or more of an index matching compound, a light absorbing compound and an antireflective compound; and
sequentially activating and coupling monomers in different selected regions of said substrate to form a plurality of different polymer sequences in different known locations on said surface of said substrate, wherein said activating step comprises directing an activation radiation at said first surface of said substrate.
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Abstract
The present invention provides novel processes for the large scale preparation of arrays of polymer sequences wherein each array includes a plurality of different, positionally distinct polymer sequences having known monomer sequences. The methods of the invention combine high throughput process steps with high resolution photolithographic techniques in the manufacture of polymer arrays.
43 Citations
98 Claims
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1. A method of forming an array of polymers on a surface of a substrate, comprising:
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providing a substrate having a first surface coated with functional groups protected with a photolabile protecting group, and a second surface having a layer disposed thereon, said layer including one or more of an index matching compound, a light absorbing compound and an antireflective compound; and
sequentially activating and coupling monomers in different selected regions of said substrate to form a plurality of different polymer sequences in different known locations on said surface of said substrate, wherein said activating step comprises directing an activation radiation at said first surface of said substrate.
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2-49. -49. (canceled)
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50. A method for synthesizing an array of chemical compounds on the surface of a support wherein said synthesis comprises a plurality of steps, said method comprising performing at least two of said steps by:
- (a) placing a support having a functionalized surface into a chamber of a flow cell and subjecting said surface to one step of said synthesis and (b) placing said support into a chamber of another flow cell and subjecting said surface to another step of said synthesis.
- View Dependent Claims (51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61)
- 62. A method for synthesizing an array of biopolymers on the surface of a support wherein said synthesis comprises a plurality of monomer additions, said method comprising after each of said monomer additions (a) placing said support into a chamber of a flow cell and subjecting said surface to a step of said synthesis that is subsequent to a monomer addition and (b) placing said support into a chamber of another flow cell and subjecting said surface to another step of said synthesis that is subsequent to step (a).
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79. An apparatus for synthesizing an array of biopolymers on the surface of a support, said apparatus comprising:
- (a) a plurality of flow cells, (b) one or more fluid dispensing stations in fluid communication with one or more of said plurality of flow cells, (c) a station for monomer addition to said surface of said support, and (d) a mechanism for moving a support to and from said station for monomer addition and a flow cell and from one flow cell to another flow cell.
- View Dependent Claims (80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92)
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93. An apparatus for synthesizing an array of biopolymers on the surface of a support, said apparatus comprising:
- (a) a plurality of flow cells wherein said flow cells comprise a chamber, a holder for said support, at least one inlet and an outlet, wherein each of said inlets is in fluid communication with a manifold and wherein said outlet is in controlled fluid communication with one or more purification systems, holding chambers and sensors, (b) one or more fluid dispensing stations in fluid communication with one or more of said plurality of flow cells by means of said manifolds, (c) a station for monomer addition to said surface of said support, (d) a mechanism for moving a support to and from said station for monomer addition and a flow cell and from one flow cell to another flow cell, and (e) a controller for controlling the movement of said mechanism.
- View Dependent Claims (94, 95, 96, 97, 98)
Specification