Methods for making a device for concurrently processing multiple biological chip assays
First Claim
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1. A method for concurrently processing multiple biological chip assays comprising the steps of:
- (a) providing a biological chip plate comprising a plurality of probe arrays and, surrounding the probe arrays, material resistant to the flow of liquid, thereby forming a plurality of test wells, each test well defining a space for the introduction of a sample;
(b) introducing into each test well test samples from a plurality of different patients, wherein each test sample contains target molecules;
(c) manipulating the biological chip plate with a fluid handling device that automatically performs steps to carry out reactions between target molecules in the test samples and probes in a plurality of the test wells, wherein the fluid handling device controls temperature, sample handling, substrate handling and washing of the test wells; and
(d) interrogating the probe arrays of the biological chip plate with a biological chip plate reader to detect reactions between target molecules and probes in a plurality of the test wells to generate assay results.
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Abstract
Methods for concurrently processing multiple biological chip assays by providing a biological chip plate comprising a plurality of test wells, each test well having a biological chip having a molecular probe array; introducing samples into the test wells, subjecting the biological chip plate to manipulation by a fluid handling device that automatically performs steps to carry out reactions between target molecules in the samples and probes; and subjecting the biological chip plate to a biological chip plate reader that interrogates the probe arrays to detect any reactions between target molecules and probes.
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Citations
80 Claims
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1. A method for concurrently processing multiple biological chip assays comprising the steps of:
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(a) providing a biological chip plate comprising a plurality of probe arrays and, surrounding the probe arrays, material resistant to the flow of liquid, thereby forming a plurality of test wells, each test well defining a space for the introduction of a sample;
(b) introducing into each test well test samples from a plurality of different patients, wherein each test sample contains target molecules;
(c) manipulating the biological chip plate with a fluid handling device that automatically performs steps to carry out reactions between target molecules in the test samples and probes in a plurality of the test wells, wherein the fluid handling device controls temperature, sample handling, substrate handling and washing of the test wells; and
(d) interrogating the probe arrays of the biological chip plate with a biological chip plate reader to detect reactions between target molecules and probes in a plurality of the test wells to generate assay results.
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2-31. -31. (canceled)
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32. A method of simultaneously conducting multiple chemical reactions in a reaction assembly that comprises a microtiter plate of wells containing test samples and an array of sets of chemical reactants comprising the steps of:
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assembling the array of sets of chemical reactants to the microtiter plate of test samples such that the array covers open ends in the test sample wells of the microtiter plate to form a plurality of closed cells, each closed cell comprising a set of chemical reactants and a respective test sample;
sealing the microtiter plate to the array to create one or more of a gas tight, a liquid tight, and a fluid tight seal; and
mechanically agitating the sealed reaction assembly to contact the test samples with the chemical reactants in each closed cell simultaneously. - View Dependent Claims (33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
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50. A method of simultaneously conducting multiple chemical reactions between a first chemical sample and a second chemical sample comprising the steps of:
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providing a plate having a plurality of wells spatially arranged in a surface of the plate in a well array pattern, each well having a side wall adjacent to a closed end that enclose the well except for an open end that is opposite the closed end and that is adjacent to the plate surface, the plurality of wells for receiving the first chemical sample via the open end;
providing an array of the second chemical sample, the ray comprising sets of the second chemical sample bound to and spatially arranged on a surface of an array substrate in an array pattern of features, the well array pattern being spatially similar to the feature array pattern;
assembling the array onto the plate to form a sealed reaction assembly, such that the surface of the array faces the surface of the plate and encloses the open ends of the plurality of wells to form closed cells, each closed cell comprising the first chemical sample and a respective set of the second chemical sample features, wherein the sealed reaction assembly is one or more of gas tight, liquid tight, and fluid tight; and
contacting the first chemical sample with the second chemical sample in each closed cell of the sealed reaction assembly. - View Dependent Claims (51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67)
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68. An apparatus for simultaneously conducting multiple chemical reactions comprising:
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a plate having a plurality of wells spatially arranged in a surface of the plate in a well array pattern, each well having a side wall adjacent to a closed end that enclose the well except for an open end that is opposite the closed end and that is adjacent to the plate surface, the plurality of wells for receiving a test sample via the open end;
an array of sets of chemical reactants, the sets of chemical reactants being bound to and spatially arranged on a surface of an array substrate in an array pattern of features, the well array pattern being spatially similar to the feature array pattern, wherein the array surface faces the plate surface and covers the open ends of the wells to form closed cells, each closed cell comprising a respective test sample and a respective set of the chemical reactants; and
a seal between the plate and the array that is one or more of gas tight, liquid tight, and fluid tight. - View Dependent Claims (69, 70, 71, 72, 73, 74, 75, 76, 77)
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78. A kit for simultaneously conducting multiple different assays of biological materials comprising:
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an array having a plurality of sets of chemical reactants spatially arranged on an may substrate; and
a plate having a plurality of spatially arranged wells in the plate, the wells being closed at one end and open at an opposite end for receiving a sample under test, wherein the array and the plate form a multiple closed cell reaction assembly when the array is assembled to the plate, such that the array covers the open ends of the wells to form closed cells, each closed cell comprising the test sample and a respective set of the chemical reactants, the reaction assembly comprising a seal between the plate and the array that is one or more of gas tight, liquid tight, and fluid tight when assembled. - View Dependent Claims (79)
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80. A method of simultaneously conducting multiple chemical reactions in a reaction assembly that comprises a microtiter plate of wells containing test samples and an may of sets of chemical reactants comprising the steps of:
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assembling the array of sets of chemical reactants to the microtiter plate of test samples such that the may covers open ends in the test sample wells of the microtiter plate to form a plurality of closed cells, each closed cell comprising a set of chemical reactants and a respective test sample, the sets of chemical reactants being bound to an array surface of the array;
sealing the microtiter plate to the array to create one or more of a gas tight, a liquid tight, and a fluid tight seal; and
mechanically agitating the sealed reaction assembly to contact the test samples with the array-bound chemical reactants in each closed cell simultaneously.
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Specification