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Genetic analysis by sequence-specific sorting

  • US 20050181408A1
  • Filed: 02/10/2005
  • Published: 08/18/2005
  • Est. Priority Date: 02/12/2004
  • Status: Active Grant
First Claim
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1. A method of sorting polynucleotides having predetermined sequence characteristics, the method comprising the steps of:

  • extending a primer annealed polynucleotides having predetermined sequence characteristics to incorporate a predetermined terminator having a capture moiety;

    capturing polynucleotides having extended primers by a capture agent that specifically binds to the capture moiety; and

    melting the captured polynucleotides from the extended primers.

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