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Method and apparatus for maintenance and expansion of hemopoietic stem cells and/or progenitor cells

  • US 20050181504A1
  • Filed: 04/11/2005
  • Published: 08/18/2005
  • Est. Priority Date: 02/04/1999
  • Status: Active Grant
First Claim
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1. A method of preparing a stromal cell conditioned medium useful in expanding undifferentiated hemopoietic stem cells to increase the number of the hemopoietic stem cells, the method comprising:

  • (a) establishing a stromal cell culture in a stationary phase plug-flow bioreactor under continuous flow on a substrate in the form of a sheet, said substrate including a non-woven fibrous matrix forming a physiologically acceptable three-dimensional network of fibers, thereby expanding undifferentiated hemopoietic stem cells; and

    (b) when a desired stromal cell density has been achieved, collecting medium from said stationary phase plug-flow bioreactor, thereby obtaining the stromal cell conditioned medium useful in expanding the undifferentiated hemopoietic stem cells.

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