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Chromosome-based platforms

  • US 20050181506A1
  • Filed: 12/06/2004
  • Published: 08/18/2005
  • Est. Priority Date: 05/30/2001
  • Status: Active Grant
First Claim
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1. A method of introducing heterologous nucleic acid into a chromosome, comprising:

  • contacting a eukaryotic chromosome comprising one or a plurality of att site(s) with a nucleic acid molecule comprising both the heterologous nucleic acid and a recombination site, in the presence of a recombinase that promotes recombination between the sites in the chromosome and in the nucleic acid molecule, wherein;

    the one or a plurality of att site(s) are heterologous to the chromosome; and

    the one or a plurality of att site(s) permits site-directed integration in the presence of lambda integrase.

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