Method and apparatus for automated image analysis of biological specimens
First Claim
1. A method, comprising:
- identifying a sample area of a sample holder, the sample area including a biological sample;
receiving image data indicative of a first portion of the sample area, the image data including first color pixel data and second color pixel data for each of a plurality of pixels;
transforming the image data to generate transformed pixel data for each of the plurality of pixels which is in a different format than said image data, and which transformed pixel data includes information indicative of both a value of the first color pixel data and a value of the second color pixel data for the associated pixel.
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Abstract
A method and apparatus for automated cell analysis of biological specimens automatically scans at a low magnification to acquire images which are analyzed to determine candidate cell objects of interest. The low magnification images are converted from a first color space to a second color space. The color space converted image is then low pass filtered and compared to a threshold to remove artifacts and background objects from the candidate object of interest pixels of the color converted image. The candidate object of interest pixels are morphologically processed to group candidate object of interest pixels together into groups which are compared to blob parameters to identify candidate objects of interest which correspond to cells or other structures relevant to medical diagnosis of the biological specimen. The location coordinates of the objects of interest are stored and additional images of the candidate cell objects are acquired at high magnification. The high magnification images are analyzed in the same manner as the low magnification images to confirm the candidate objects of interest which are objects of interest. A high magnification image of each confirmed object of interest is stored for later review and evaluation by a pathologist.
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Citations
32 Claims
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1. A method, comprising:
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identifying a sample area of a sample holder, the sample area including a biological sample;
receiving image data indicative of a first portion of the sample area, the image data including first color pixel data and second color pixel data for each of a plurality of pixels;
transforming the image data to generate transformed pixel data for each of the plurality of pixels which is in a different format than said image data, and which transformed pixel data includes information indicative of both a value of the first color pixel data and a value of the second color pixel data for the associated pixel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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17. An article comprising a machine-readable medium embodying information indicative of instructions that when performed by one or more machines result in operations comprising:
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automatically identifying a sample area of a sample holder, the sample area including a biological sample;
receiving image data indicative of a first portion of the sample area, the image data including first color pixel data and second color pixel data for each of a plurality of pixels;
transforming the image data to generate transformed pixel data for each of the plurality of pixels which is in a different format than said image data, and which transformed pixel data includes information indicative of both a value of the first color pixel data and a value of the second color pixel data for the associated pixel. - View Dependent Claims (18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification