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Methods and compositions for detecting nucleic acids

  • US 20050186606A1
  • Filed: 02/11/2005
  • Published: 08/25/2005
  • Est. Priority Date: 02/11/2004
  • Status: Abandoned Application
First Claim
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1. A reporter molecule for detecting a nucleic acid, the molecule comprising:

  • an enzyme having a kcat of at least about 200 sec

    1
    ;

    a reversible inhibitor of said enzyme inhibitorily engaging said enzyme; and

    a nucleobase polymer extending between said enzyme and said reversible inhibitor;

    wherein said polymer is operable to interfere with the engagement between said inhibitor and said enzyme when said nucleic acid contacts said polymer.

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