Method for the production of transgenic proteins useful in the treatment of obesity and diabetes
First Claim
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1. A transgenically produced recombinant protein, encoded by a transgene DNA construct comprising a polypeptide domain which has a desired bioactivity comprising said transgenic protein or fragment thereof wherein said polypeptide domain retains its endogenous physiological activity and is useful in reducing the body-weight of a mammal.
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Abstract
Transgenic proteins therapeutically useful in the treatment of obesity and related conditions can be produced in and purified from the milk of transgenic animals. The peptides are made as transgenic proteins with a suitable transgenic partner such as human recombinant protein of interest.
19 Citations
43 Claims
- 1. A transgenically produced recombinant protein, encoded by a transgene DNA construct comprising a polypeptide domain which has a desired bioactivity comprising said transgenic protein or fragment thereof wherein said polypeptide domain retains its endogenous physiological activity and is useful in reducing the body-weight of a mammal.
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25. A method for the production of transgenic animals capable of producing a transgenic protein of interest comprising:
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transfecting a non-human mammalian cell-line with a transgene DNA construct encoding a desired transgenic protein;
selecting a cell line(s) in which said transgene DNA construct has been inserted into the genome of that cell or cell-line; and
performing a first nuclear transfer procedure to generate a first transgenic animal heterzygous for the desired gene said animal being capable of expressing said transgenic protein of interest in its milk. - View Dependent Claims (26, 27, 28, 29)
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30. A method for treating a human disease or pathologic condition related to or caused by obesity with a composition of matter containing a recombinant transgenic protein, said transgenic protein encoding a human recombinant protein selected from the group consisting of:
- Leptin;
Glucagon;
subpeptide PYY;
α
-MSH;
a dysfunctional Ghrelin Receptor;
a dysfunctional MC-1 Receptor;
a dysfunctional NPY Y1 Receptor;
a dysfunctional NPY Y5 Receptor;
a dysfunctional GAL1 Receptor;
a dysfunctional GAL2 Receptor;
a dysfunctional GAL3 Receptor;
a dysfunctional Orexin Receptor 1; and
a dysfunctional Orexin Receptor 2. - View Dependent Claims (31, 32, 40)
- Leptin;
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33. A method for treating a human disease related to undesirably low body weight or weight loss with a composition of matter containing a recombinant transgenic protein, said transgenic protein encoding a human recombinant protein selected from the group consisting of:
- a dysfunctional Leptin receptor;
Ghrelin;
a dysfunctional glucagons Receptor;
Agouti Signalling Protein;
a dysfunctional MC-4 Receptor;
NPY;
Agouti Related Protein (AGRP);
a dysfunctional MC-3 Receptor;
Galanin;
an Orexin A polypeptide; and
an Orexin B polypeptide. - View Dependent Claims (34, 41)
- a dysfunctional Leptin receptor;
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35. A method for treating a human disease or pathologic condition related to or caused by obesity with two proteins, wherein a first protein is recombinant leptin and a second protein is a composition of matter containing a recombinant transgenic protein, said second protein is a human recombinant protein selected from the group consisting of:
- Glucagon;
subpeptide PYY;
α
-MSH;
a dysfunctional Ghrelin Receptor;
a dysfunctional MC-1 Receptor;
a dysfunctional NPY Y1 Receptor;
a dysfunctional NPY Y5 Receptor;
a dysfunctional GAL1 Receptor;
a dysfunctional GAL2 Receptor;
a dysfunctional GAL3 Receptor;
a dysfunctional Orexin Receptor 1; and
a dysfunctional Orexin Receptor 2. - View Dependent Claims (36, 37, 42)
- Glucagon;
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38. A method for treating a human disease related to undesirably low body weight or weight loss with two recombinant proteins, wherein a first protein is recombinant ghrelin and a second protein is a human recombinant protein selected from the group consisting of:
- a dysfunctional Leptin receptor;
a dysfunctional glucagons Receptor;
Agouti Signalling Protein;
a dysfunctional MC-4 Receptor;
NPY;
Agouti Related Protein (AGRP);
a dysfunctional MC-3 Receptor;
Galanin;
an Orexin A polypeptide; and
an Orexin B polypeptide. - View Dependent Claims (39, 43)
- a dysfunctional Leptin receptor;
Specification