Device and method for multiple analyte detection

• US 20050186684A1
• Filed: 04/12/2005
• Published: 08/25/2005
• Est. Priority Date: 04/03/1996
• Status: Abandoned Application

• Current Assignee: Applied Biosystems LLC (Thermo Fisher Scientific Incorporated)
• Original Assignee: Applera Corporation (Thermo Fisher Scientific Incorporated)
• Inventors: Kowallis, Reid B., Albin, Michael, Winn-Deen, Emily S., Ragusa, Robert P., Raysberg, Yefim, Woudenberg, Timothy M.
• Application Number:
  US11/104,156
• Publication Number:
  US 20050186684A1
• Time in Patent Office: Days
• US Class Current: 436/174
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00599 Solution-phase processes
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/0642 Filling fluids into wells by specific techniques
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/08 using multifield media
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2565/631 being a biochannel or pore
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 50/08 Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 2035/00148 Test cards, e.g. Biomerieux or McDonnel multiwell test cards
• CPC Class Codes: G01N 21/6452 : Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: Y10S 436/805 : Optical property
• CPC Class Codes: Y10S 436/807 : Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 436/808 : Automated or kit
• CPC Class Codes: Y10S 436/809 : Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/25 : including sample preparation

Abstract

A method for making a test device is provided. The method can comprise providing (a) a substrate comprising a plurality of wells, wherein each well comprises at least one dimension no greater than 1 mm; (ii) a robotic dispensing system comprising at least one piezoelectric dispenser; and (iii) a liquid solution comprising at least one analyte-specific reagent. The method further comprises delivering a volume of the liquid solution into at least some of the wells using the at least one piezoelectric dispenser. The volume can be no greater than about 1 microliter. The method can further comprise placing a cover over the wells.

Claims

1. A method for making a test device, comprising:

providing (a) a substrate comprising a plurality of wells, wherein each well comprises at least one dimension no greater than 1 mm;

(ii) a robotic dispensing system comprising at least one piezoelectric dispenser; and

(iii) a liquid solution comprising at least one analyte-specific reagent;

delivering a volume of said liquid solution into at least some of said wells, using said at least one piezoelectric dispenser, wherein said volume is no greater than about 1 microliter; and

placing a cover over said wells.

2. The method of claim 1, further comprising, prior to said placing, at least partially drying said liquid solution.

3. The method of claim 2, wherein said drying is effected, at least in part, by baking.

4. The method of claim 2, wherein said drying is effected, at least in part, by reduced pressure.

5. The method of claim 1, wherein said cover seals said wells.

6. The method of claim 1, wherein said cover is optically transparent.

7. The method of claim 1, wherein each of said wells comprises at least one dimension no greater than 0.2 mm.

8. The method of claim 1, wherein said substrate comprises at least 49 wells within an area no greater than 3 cm×

2 cm.

9. The method of claim 1, wherein said at least one reagent comprises at least two oligonucleotides primers and a detectable probe.

10. The method of claim 1, wherein said robotic dispensing system comprises a plurality of piezoelectric dispensers.