Anti-stokes raman in vivo probe of analyte concentrations through the human nail
First Claim
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1. A method for in vivo detection of an analyte, comprising the steps of:
- illuminating a sample volume of body tissue with a beam of optical radiation at an incident wavelength from an optical source;
collecting scattered anti-Stokes Raman radiation emitted from within the sample volume;
analyzing the collected scattered anti-Stokes Raman radiation to determine a intensity response as a function of wavelength;
calculating the analyte concentration based on the intensity response as a function of wavelength.
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Abstract
A system and method are provided for detecting and quantifying an analyte in vivo. Anti-Stokes Raman scattered radiation emitted from a sample under incident radiation excitation is collected and analyzed. The intensity response is corrected for temperature effects using a Boltzmann correction factor based on the temperature of the sample. The sampled tissue is advantageously the sterile matrix beneath the nail of either a toe or a finger. The incident excitation radiation is projected onto the sterile matrix through the nail, which operates as a window. The present invention may be applied in both the blue/UV and the red/IR regions of the spectrum.
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Citations
24 Claims
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1. A method for in vivo detection of an analyte, comprising the steps of:
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illuminating a sample volume of body tissue with a beam of optical radiation at an incident wavelength from an optical source;
collecting scattered anti-Stokes Raman radiation emitted from within the sample volume;
analyzing the collected scattered anti-Stokes Raman radiation to determine a intensity response as a function of wavelength;
calculating the analyte concentration based on the intensity response as a function of wavelength. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A system for using anti-Stokes Raman spectography to detect an analyte in vivo, comprising:
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a digit holder for positioning a digit, the digit comprising skin and a nail plate, the nail plate having a first end that is under the skin and a second opposite end disposed proximate to a tip of the digit, the digit holder comprising a substantially flat base plate attached to a back wall, the back wall being disposed approximately perpendicularly to the base plate, such that the digit may be placed in the holder with a side of the digit opposite to the nail plate resting on the base plate and the second end of the nail plate disposed proximate to the back wall;
a sensor for measuring the temperature of the digit, the sensor being attached to the digit holder;
an incident light source, the incident light source providing excitation radiation at an excitation wavelength, the excitation radiation being directed through the nail plate into a sterile matrix beneath the nail plate; and
a collection subsystem, the collection subsystem receiving scattered radiation emitted within the sterile matrix. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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23. A method for in vivo detection of an analyte, comprising the steps of:
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projecting excitation light onto a nail of a digit to illuminate a sample volume under the nail;
measuring the temperature of the digit;
collecting Raman scattered light emitted from the sample volume, the Raman scattered light comprising an anti-Stokes signal;
processing the Raman spectrum of the scattered light to quantify one or more peak metrics for the anti-Stokes signal;
correcting the peak metrics based on a Boltzmann correction factor, the Boltzmann correction factor being calculated using the measured temperature of the digit; and
determining the concentration of the analyte based on a partial least squares analysis using the Boltzmann-adjusted peak metrics. - View Dependent Claims (24)
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Specification