Microfluidic devices for electrophoretic analysis of materials
First Claim
1. A method of separating components of a plurality of subject materials in a microfluidic system, the method comprising:
- flowing a first fluid comprising a series of a plurality of subject materials through a first microchannel to a separation channel by applying pressure to the first fluid wherein the pressure is applied to the first fluid by a pressure source external to the first microchannel; and
applying an electrophoretic force to the first fluid to introduce individual ones of the plurality of subject materials into the separation channel, and to separate each of the plurality of subject materials into its respective sample components.
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Abstract
The present invention provides a microfluidic system for fast, accurate and low cost electrophoretic analysis of materials in the fields of chemistry, biochemistry, biotechnology, molecular biology and numerous other fields. Light from periodically spaced regions along a channel in the microfluidic system are received by a photodetector. The intensity of light received by the photodetector is modulated by the movement of species bands through the channel under electrophoretic forces. By Fourier analysis, the velocity of each species band is determined and the identification of the species is made by its electrophoretic mobility in the channel.
49 Citations
24 Claims
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1. A method of separating components of a plurality of subject materials in a microfluidic system, the method comprising:
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flowing a first fluid comprising a series of a plurality of subject materials through a first microchannel to a separation channel by applying pressure to the first fluid wherein the pressure is applied to the first fluid by a pressure source external to the first microchannel; and
applying an electrophoretic force to the first fluid to introduce individual ones of the plurality of subject materials into the separation channel, and to separate each of the plurality of subject materials into its respective sample components. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A device for separating components of a fluid, the device comprising:
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a microfluidic device comprising a body structure comprising at least first and second intersecting microchannels fabricated therein;
a pressure source fluidly coupled to the first microchannel; and
a voltage or current controller operably coupled to the second microchannel;
wherein the first microchannel is fluidly coupled to at least one source of a plurality of different subject materials. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification