Tangential flow filtration devices and methods for stem cell enrichment
First Claim
Patent Images
1. A method for separating stem cells from a sample from a subject wherein the sample comprises stem cells and non-stem cell constituents, the method comprising separation on a tangential flow filtration device by:
- (i) introducing the sample into a remover unit (1) comprising a cross-flow chamber (3) through an inlet (6) in the remover unit;
(ii) subjecting the sample to cross-flow substantially parallel to a filter (5) having a pore size of about 1 to about 10 microns;
(iii) subjecting the fluid to filtration through the filter; and
(iv) selectively removing non-stem cell constituents from the sample to form a cell population enriched for stem cells.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides methods for enriching a heterogenous mixture of bone marrow or blood constituents for stem cells by removal of non-stem cell constituents comprising separation of the non-stem cell constituents using a tangential flow filtration device.
-
Citations
43 Claims
-
1. A method for separating stem cells from a sample from a subject wherein the sample comprises stem cells and non-stem cell constituents, the method comprising separation on a tangential flow filtration device by:
-
(i) introducing the sample into a remover unit (1) comprising a cross-flow chamber (3) through an inlet (6) in the remover unit;
(ii) subjecting the sample to cross-flow substantially parallel to a filter (5) having a pore size of about 1 to about 10 microns;
(iii) subjecting the fluid to filtration through the filter; and
(iv) selectively removing non-stem cell constituents from the sample to form a cell population enriched for stem cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
-
-
27. A method for enriching a sample of bone marrow or blood constituents for stem cells, comprising:
-
(i) introducing the sample into a tangential flow filtration (TFF) unit, the TFF unit comprising a cross-flow chamber, a filtrate chamber, and a filter in fluid communication with the cross-flow chamber and the filtrate chamber, the filter having a pore size of about 1 to about 10 microns;
(ii) recirculating the sample through the TFF unit at a predetermined input rate and a predetermined filtration rate, the predetermined input rate at least five times the predetermined filtration rate;
wherein the predetermined filtration rate is less than the unopposed filtration rate for the filter; and
(iii) isolating a cell population enriched for stem cells. - View Dependent Claims (28)
-
Specification