Cultures of GFAP nestin cells that differentiate to neurons
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Abstract
Cultures of cells immunoreactive for glial fibrillary acidic protein (GFAP), as well as for the intermediate filament marker nestin were grown in a medium including epidermal growth factor (EGF) and serum. The cultured cells had the morphology of astroglial cells. The cells can be proliferated in adherent or suspension cultures. Depending on the culture conditions, the cells can be induced to differentiate to neurons or glial cells. The cultures can be expanded over a large number of passages during several months, and survive, express an astroglial phenotype and integrate well after transplantation into both neonatal and adult rat forebrain.
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Citations
74 Claims
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1-56. -56. (canceled)
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57. An in vitro cell culture of GFAP+ nestin+ cells, wherein
a) one or more cells in the culture have the capacity to differentiate into neurons; -
b) the cell culture divides in a culture medium containing at least one proliferation-inducing growth factor; and
c) one or more cells in the culture differentiate into neurons upon withdrawal of the proliferation-inducing growth factor. - View Dependent Claims (58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74)
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Specification