Method of treatment using electroporation mediated delivery of drugs and genes
0 Assignments
0 Petitions
Accused Products
Abstract
A method for in vivo electrotherapy, or electroporation-mediated therapy, using a needle array apparatus is provided. Treatment of tumors with a combination of electroporation using the apparatus of the invention, and a chemotherapeutic agent, caused regression of tumors in vivo.
68 Citations
36 Claims
-
1. canceled
-
2. A method of electroporating a portion of the body of a patient to introduce molecules into tissues therein, comprising:
-
a. providing an array of multiple opposed pairs of electrodes connected to a generator, wherein at least two pairs of electrodes are activated simultaneously, at least one of said pairs of electrodes having a needle configuration for penetrating tissue;
b. inserting a first pair of said needle electrodes, into selected tissue for introducing molecules into the tissue;
c. positioning a second pair of electrodes of said array of electrodes in conductive relation to said selected tissue so that said tissue is between said first and second opposed pairs of electrodes; and
d. applying electric pulses to at least two different pairs of electrodes to generate an electric field of sufficient strength to cause in vivo electroporation of the tissue. - View Dependent Claims (7, 8, 17, 18)
-
-
3. A method for the delivery of molecules to a predetermined tissue of a subject, comprising:
-
a. providing an array of electrodes comprising at least two pairs of opposed electrodes, wherein at least one of said electrodes has a needle configuration for penetrating said tissue;
b. contacting said tissue with said array such that the electrode(s) penetrate said tissue;
C. administering said molecules to said tissue; and
d. applying at least one electric pulse simultaneously to at least two of the pairs of electrodes sufficient to electroporate cells of said tissue and thereby allow said molecules to be delivered to said tissue. - View Dependent Claims (4, 5, 6)
-
-
9. A method for the delivery of molecules to a predetermined tissue of a subject, comprising:
-
a. providing an array of electrodes comprising at least two pairs of opposed electrodes, wherein at least one of said electrodes has a needle configuration for penetrating said tissue;
b. contacting said tissue with said array such that the electrode(s) penetrate said tissue;
c. administering said molecules to said tissue;
d. applying at least one electric pulse simultaneously to at least two of the pairs of opposed electrodes sufficient to electroporate cells of said tissue using an electric field of predefined strength and thereby allow said molecules to be delivered to said tissue. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
-
-
19. A method for the delivery of molecules to a predetermined tissue of a subject, comprising:
-
a. providing an electrode apparatus for the application of at least one electric pulse, wherein said electrode apparatus comprises;
i. at least two pairs of needles for penetrating said predetermined tissue, wherein each needle has a lumen for insertion of an electrode;
ii. at least two pairs of electrodes, wherein each electrode is configured for insertion into the tissue through the lumen of one of said needles; and
iii. a power generator to apply an electric potential of sufficient strength across at least two of the pairs of the electrodes to generate an electric field for electroporation of cells of the tissue; and
b. applying at least one electric pulse simultaneously to at least two of the pairs of electrodes sufficient to electroporate cells of said tissue and thereby allow said molecules to be delivered to said tissue. - View Dependent Claims (20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34)
-
-
35. A method for the delivery of molecules to a predetermined tissue of a subject, comprising:
-
a. providing an electrode apparatus for the application of at least one electric pulse, wherein said electrode apparatus comprises;
i. at least two pairs of needle electrodes for penetrating said predetermined tissue, wherein at least one of said needle electrodes is used to deliver a solution containing said molecules to said tissue, and wherein at least two pairs of said needle electrodes is used to deliver at least one electric impulse; and
ii. a power generator to apply an electric potential of sufficient strength across at least two of the pairs of the electrodes to generate an electric field for electroporation of cells of the tissue; and
b. applying at least one electric pulse simultaneously to at least two of the pairs of electrodes sufficient to electroporate cells of said tissue and thereby allow said molecules to be delivered to said tissue. - View Dependent Claims (36)
-
Specification