Somatic cell line
0 Assignments
0 Petitions
Accused Products
Abstract
The invention features a purified embryonic or fetal caprine somatic cell. The somatic cell can be in a preparation of embryonic or fetal caprine somatic cells. In addition, the cell can be used to derive an embryonic or fetal caprine somatic cell line. The somatic cell can be a genetically engineered caprine somatic cell, e.g., a somatic cell which includes a transgenic sequence, or which a nucleic acid has been introduced.
-
Citations
130 Claims
-
1-91. -91. (canceled)
-
92. A method for the accelerated production of transgenic animals comprising:
-
a) transfecting a first non-human differentiated somatic cell or cell-line with a transgene construct containing a first DNA sequence;
b) selecting a transfected cell or cell-line into which said first DNA sequence has been inserted into the genome of said first non-human differentiated somatic cell or cell-line;
c) performing a first nuclear transfer procedure to generate a first transgenic animal at least heterozygous for said first DNA sequence;
d) performing a biopsy or other cell selection technique to obtain cells to establish a second non-human differentiated somatic cell or cell-line from said first transgenic animal;
e) characterizing said second non-human differentiated somatic cell or cell-line using known molecular biology methods to ensure that the selected said second non-human differentiated somatic cell or cell-line is at least heterozygous for said first DNA sequence; and
f) performing a second nuclear transfer procedure with at least one of said second non-human differentiated somatic cells to produce at least a second transgenic animal at least heterozygous for said first DNA sequence. - View Dependent Claims (93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 106, 107, 108, 109, 110, 111, 112, 113, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130)
-
-
104. A method of preparing a genetically engineered transgenic mammal, comprising:
-
(a) inseminating a first female non-human mammal recipient with semen from a transgenic non-human animal of the same species known to have a transgene present and expressed;
(b) obtaining a transgenic non-human embryo from said first female recipient;
(c) obtaining a somatic cell from said embryo;
(d) culturing said differentiated somatic cell in a suitable medium, such that a differentiated somatic cell line is obtained and, (e) performing a nuclear transfer procedure with said non-human differentiated somatic cells to produce at least one transgenic mammal at least heterozygous for said first DNA sequence;
wherein said first DNA sequence encoding a desired gene is actuated by a tissue specific promoter. - View Dependent Claims (105, 114, 115, 116, 117, 118)
-
Specification