Lesion repair polymerase compositions
First Claim
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1. A composition comprising at least one lesion repair polymerase and at least one second polymerase.
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Abstract
Compositions comprising at least two polymerases, including a lesion repair polymerase, are provided. Methods for producing primer extension products using at least two polymerases, including a lesion repair polymerase, are also provided. Kits for producing primer extension products comprising at least two polymerases, including a lesion repair polymerase, are also provided.
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Citations
204 Claims
- 1. A composition comprising at least one lesion repair polymerase and at least one second polymerase.
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31. A method of amplifying a lesion-containing target nucleic acid comprising incubating the lesion-containing target nucleic acid with at least one primer, at least one extendable nucleotide, at least one lesion-repair polymerase, and at least one second polymerase under conditions to generate at least one primer extension product.
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32-56. -56. (canceled)
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57. A method of sequencing a lesion-containing target nucleic acid comprising:
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(a) incubating the lesion-containing target nucleic acid with at least one primer, at least one extendable nucleotide, at least one terminator, at least one lesion repair polymerase, and at least one second polymerase, under conditions to generate at least one primer extension product comprising a terminator;
(b) separating the at least one primer extension product comprising a terminator;
(c) detecting at least one of the at least one primer extension product comprising a terminator; and
(d) determining the sequence of the lesion-containing target nucleic acid.
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58-84. -84. (canceled)
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85. A method of genotyping a lesion-containing target nucleic acid comprising:
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(a) incubating the lesion-containing target nucleic acid with at least one primer, at least one extendable nucleotide, at least one lesion repair polymerase, and at least one second polymerase, under conditions to generate at least one primer extension product;
(b) separating the at least one primer extension product;
(c) detecting the at least one primer extension product; and
(d) determining the genotype of the lesion-containing target nucleic acid.
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86-194. -194. (canceled)
- 195. A kit comprising at least one lesion repair polymerase and at least one second polymerase.
Specification