Guided deposition in spatial arrays
First Claim
1. A method for conducting a plurality of reactions on a substrate, said substrate having a plurality of cells at predefined locations thereon, the location of each cell being encoded by information readable with electrical, magnetic or electromagnetic sensing means, said method comprising the following steps:
- (a) selecting a first set of cells;
(b) decoding the locations of said first set of cells by said sensing means and delivering a first reactant to each of said first set cells;
(c) selecting a second set of cells;
(d) decoding the locations of said second set of cells by said sensing means and delivering a second reactant to each of said second set of cells; and
(e) simultaneously reacting said first reactant in each of said first set of cells and said second reactant in each of said second set of cells, wherein the first reactant is prevented from contacting the second set of cells and the second reactant is prevented from contacting the first set of cells.
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Abstract
An apparatus and method is provided for preparing and using a very large and diverse array of compounds on a substrate having rapidly accessible locations. The substrate contains cells in which the compounds of the array are located. Surrounding the cells is a non-wetable surface that prevents the solution in one cell from moving to adjacent cells. The compounds are delivered to the individual cells of the array by a micropipette attached to an X-Y translation stage.
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Citations
54 Claims
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1. A method for conducting a plurality of reactions on a substrate, said substrate having a plurality of cells at predefined locations thereon, the location of each cell being encoded by information readable with electrical, magnetic or electromagnetic sensing means, said method comprising the following steps:
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(a) selecting a first set of cells;
(b) decoding the locations of said first set of cells by said sensing means and delivering a first reactant to each of said first set cells;
(c) selecting a second set of cells;
(d) decoding the locations of said second set of cells by said sensing means and delivering a second reactant to each of said second set of cells; and
(e) simultaneously reacting said first reactant in each of said first set of cells and said second reactant in each of said second set of cells, wherein the first reactant is prevented from contacting the second set of cells and the second reactant is prevented from contacting the first set of cells.
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2-20. -20. (canceled)
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21. An apparatus for assaying biological materials comprising:
- a substrate having a surface, an outer edge and a center; and
a plurality of discrete features bound to an annular region on the surface of the substrate, the plurality of discrete features comprising a first biological material. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
- a substrate having a surface, an outer edge and a center; and
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34. A system for synthesizing an annular array of biological materials comprising:
- a holder for holding a substrate;
a spinner assembly for rotating the holder;
an enclosure for enclosing at least the holder and substrate;
a deposition assembly for depositing biological material onto a surface of the substrate;
a dispenser assembly for providing ancillary materials to the surface of the substrate; and
a controller for automatically controlling the deposition assembly, the spinner assembly and the dispenser assembly. - View Dependent Claims (35, 36, 37)
- a holder for holding a substrate;
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38. A system for hybridizing biological materials in an annular array pattern comprising:
- a holder for holding a substrate, the substrate having an array of first biological material bound to an annular region on a surface of the substrate;
a spinner assembly for rotating the holder at a plurality of rotational speeds, the holder being connected to the assembly;
a deposition tool for providing a second biological material onto the surface of the substrate; and
a dispenser tool for dispensing ancillary materials into the chamber, wherein the spinner assembly rotates the holder and the substrate at least one of the plurality of rotational speeds after the second biological material is provided and at least another one of the plurality of rotational speeds after the ancillary materials are dispensed. - View Dependent Claims (39, 40, 41)
- a holder for holding a substrate, the substrate having an array of first biological material bound to an annular region on a surface of the substrate;
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42. A system for optically interrogating an annular array of hybridized biological material comprising:
- a light source for emitting a light beam;
a first optics subsystem for directing the light beam onto the array;
a scanning subsystem for providing rotational movement to the array during an optical scan;
a detector subsystem for detecting a signal from the array in response to the light beam; and
an analysis subsystem for analyzing the detected signals gathered by the detection subsystem and for automatically controlling the light source, the scanning subsystem and the detector subsystem. - View Dependent Claims (43, 44, 45, 46, 47)
- a light source for emitting a light beam;
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48. A method of assaying biological material in an annular array comprising the steps of:
- providing a plurality of discrete features comprising a first biological material in an annular region on a surface of a substrate;
hybridizing a second biological material with the first biological material on the substrate, the hybridized discrete feature having a signal producing system;
optically interrogating the hybridized discrete features on the substrate; and
determining characteristics about the first biological material and the second biological material from the optical interrogation. - View Dependent Claims (49, 50, 51, 52, 53, 54)
- providing a plurality of discrete features comprising a first biological material in an annular region on a surface of a substrate;
Specification