High-density ion transport measurement biochip devices and methods
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Accused Products
Abstract
The present invention provides novel biochips, biochip-based devices, and device configurations that can be used for ion transport measurement. The chips, devices, and designs of the present invention are particularly suited to high-throughput assays such as compound screening assays using patch clamping techniques. The invention includes high-density biochips made by novel methods and methods of making high density biochips, and also provides novel upper chamber configurations and fluidics designs for upper chambers of ion transport measurement devices that can be used in high throughput patch clamp assays. The present invention also includes methods of using ion transport measuring chips and devices of the present invention.
143 Citations
223 Claims
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1-174. -174. (canceled)
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175. A hydrophilic biochip for ion transport measurement comprising a substrate that comprises:
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one or more holes;
one or more hydrophilic recording site areas, wherein each of said one or more hydrophilic recording site areas on the particle-sealing side of said biochip; and
at least one hydrophobic area comprising the surface of said substrate surrounding said at least one hydrophilic recording site area;
wherein said at least one hydrophobic area can maintain an aqueous solution localized to said nonhydrophobic recording site area in fluid isolation. - View Dependent Claims (176, 177, 178, 179, 180, 181, 182, 183, 184, 185, 186, 187, 188)
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189. A method of making a hydrophilic chip, comprising:
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providing a substrate that comprises a hydrophilic material;
coating said substrate with at least one hydrophobic material;
making at least one hole through said substrate; and
removing said hydrophobic substrate from an area immediately surrounding said at least one hole. - View Dependent Claims (190, 191, 192)
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193. A method of making a hydrophilic chip, comprising:
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providing a substrate that comprises a hydrophilic material;
making at least one hole through said substrate; and
coating at least a portion of said substrate with at least one hydrophobic material, wherein from an area immediately surrounding said at least one hole is masked to prevent it from receiving said coating.
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194. A method of making a hydrophilic chip, comprising:
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providing a substrate that comprises a hydrophobic material;
making at least one hole through said substrate; and
coating an area immediately surrounding said at least one hole with at least one hydrophilic material. - View Dependent Claims (195, 196)
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197. A method of making an ion transport measurement microchannel plate (MCP), comprising:
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a) providing an MCP comprising at least two microchannels;
b) chemically treating at least one surface of said microchannel plate or a portion thereof to increase the electrical sealing properties of said at least two microchannels.
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198. A method of making a flexible chip for ion transport measurement, comprising:
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a) providing a substrate comprising at least one flexible material; and
b) making at least one hole through said substrate to make a flexible ion transport measuring chip. - View Dependent Claims (199, 200, 201, 202, 203, 204, 205, 206, 207, 208, 209, 210, 211, 212)
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213. A method of making an ion transport measuring device, comprising:
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a) providing at least two theta tubing segments, wherein each theta tubing segment comprises an upper compartment and a lower compartment separated by a glass septum;
b) cutting openings in the tops of said at least two theta tubing segments to provide access to the upper compartments of said at least two theta tubing segments;
c) using said openings to laser drill or etch at least one hole through the glass septum of each of said at least two theta tubing segments;
d) sealing said openings in the tops of said theta tubing segments after laser drilling or etching said at least one hole;
e) attaching said at least two theta tubing segments on top of one another or side-by-side; and
f) attaching conduits to said upper compartments and said lower compartments.
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214. A device for ion transport measurement, comprising:
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a) a chip comprising one or more ion transport measuring holes; and
b) one or more upper chambers situated above said chip such that each of said one or more upper chambers is accessed by at least one of said one or more ion transport measuring holes;
further wherein said at least one upper chamber comprises at least two openings, wherein at least one of said at least two openings is at least one inlet on one side of said at least one ion transport measuring hole, and at least one other of said at least two openings is at least one outlet on the opposite side of said at least one ion transport measuring hole. - View Dependent Claims (215, 216, 217, 218, 219, 220, 221, 222, 223)
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Specification