Self-contained microfluidic biochip and apparatus
First Claim
1. A method of gene expression profiling or screening of candidate genes for a genetic study comprising steps of:
- a) procurement means for simultaneous nucleic acid synthesis;
b) treatment means for nucleic acid amplification and labeling of at least one nucleic acid element; and
c) analyzing means for at least single-color hybridization to an integrated array of spotted bio-molecules where all three means are performed in a self-contained disposable microfluidic biochip apparatus, the apparatus comprising;
a body structure comprising a plurality of reagent cavities and at least one reaction well connected via microfluidic channels, said reagent cavities each storing a reagent and each comprising a breakable seal allowing said reagent to be selectively released into said reaction well upon being punctured.
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Abstract
A biochip and apparatus is disclosed for performing biological assays in a self-contained microfluidic platform. The disposable biochip for multi-step reactions comprises a body structure with a plurality of reagent cavities and reaction wells connected via microfluidic channels; the reagent cavities with reagent sealing means for storing a plurality of reagents; the reagent sealing means being breakable and allowing a sequence of reagents to be released into microfluidic channel and reaction well; and the reaction well allowing multi-step reactions to occur by sequentially removing away the residual reagents. The analysis apparatus can rapidly, automatically, sensitively, and simultaneously detect and identify multiple analytes or multiple samples in a very small quantity.
71 Citations
19 Claims
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1. A method of gene expression profiling or screening of candidate genes for a genetic study comprising steps of:
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a) procurement means for simultaneous nucleic acid synthesis;
b) treatment means for nucleic acid amplification and labeling of at least one nucleic acid element; and
c) analyzing means for at least single-color hybridization to an integrated array of spotted bio-molecules where all three means are performed in a self-contained disposable microfluidic biochip apparatus, the apparatus comprising;
a body structure comprising a plurality of reagent cavities and at least one reaction well connected via microfluidic channels, said reagent cavities each storing a reagent and each comprising a breakable seal allowing said reagent to be selectively released into said reaction well upon being punctured. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification