Method of determining an analyte concentration in a sample from an absorption spectrum
First Claim
1. A method of determining an analyte concentration in a biological sample, the sample comprising the analyte, water, and a protein, the method comprising:
- providing an absorption spectrum of the sample, the absorption spectrum having an absorption baseline;
shifting the absorption spectrum so that the absorption baseline approximately equals a selected absorption value in a selected absorption wavelength range at or near an isobestic wavelength at which both water and the protein have substantially the same absorbance; and
subtracting a water contribution from the absorption spectrum, thereby providing a corrected absorption spectrum substantially free of a contribution from the water.
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Abstract
A method determines an analyte concentration in a sample including the analyte and a substance. The method includes providing an absorption spectrum of the sample. The absorption spectrum has an absorption baseline. The method further includes shifting the absorption spectrum so that the absorption baseline approximately equals a selected absorption value in a selected absorption wavelength range. The method further includes subtracting a substance contribution from the absorption spectrum. Thus, the method provides a corrected absorption spectrum substantially free of a contribution from the substance.
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Citations
37 Claims
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1. A method of determining an analyte concentration in a biological sample, the sample comprising the analyte, water, and a protein, the method comprising:
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providing an absorption spectrum of the sample, the absorption spectrum having an absorption baseline;
shifting the absorption spectrum so that the absorption baseline approximately equals a selected absorption value in a selected absorption wavelength range at or near an isobestic wavelength at which both water and the protein have substantially the same absorbance; and
subtracting a water contribution from the absorption spectrum, thereby providing a corrected absorption spectrum substantially free of a contribution from the water. - View Dependent Claims (2, 3, 4, 5, 9, 13, 14, 16, 17, 18, 19, 20, 24, 25, 26, 27, 28)
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6. (canceled)
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15. (canceled)
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21. (canceled)
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23. (canceled)
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29. A method of providing pathlength-insensitive measurements of blood constituents in a sample using infrared (IR) spectroscopy, the method comprising:
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providing an absorption spectrum of the sample, the absorption spectrum having an absorption baseline; and
shifting the absorption spectrum so that the absorption baseline approximately equals a selected absorption value in an absorption wavelength range comprising an isosbestic wavelength at which water and a whole blood protein have approximately equal absorptions.
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30. (canceled)
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31. (canceled)
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32. A method of determining an analyte concentration in a biological sample, the method comprising:
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providing a cuvette;
filling the cuvette with saline and measuring a reference absorbance spectrum of the cuvette containing the saline;
filling the cuvette with the biological sample and measuring a sample absorbance spectrum of the cuvette containing the biological sample; and
calculating the analyte concentration in the biological sample using the reference absorbance spectrum and the sample absorbance spectrum. - View Dependent Claims (33, 34, 35, 36, 37)
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Specification