Method and apparatus for imaging a sample on a device
First Claim
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1. An apparatus for imaging a sample located on a support, said apparatus comprising:
- a body for immobilizing said support, said support comprising at least a first surface having said sample thereon;
an electromagnetic radiation source for generating excitation radiation having a first wavelength;
excitation optics for transforming the geometry of said excitation radiation to a line and directing said line at said sample for exciting a plurality of regions thereon, said line causing a labeled material on said sample to emit response radiation, said response radiation having a second wavelength, said first wavelength different from said second wavelength;
collection optics for collecting said response radiation from said plurality of regions;
a detector for sensing said response radiation received by said collection optics, said detector generating a signal proportional to the amount of radiation sensed thereon, said signal representing an image associated with said plurality of regions from said sample;
a translator coupled to said body for allowing a subsequent plurality of regions on said sample to be excited;
a processor for processing and storing said signal so as to generate a 2-dimensional image of said sample; and
a focuser for automatically focusing said sample in a focal plane of said excitation radiation.
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Abstract
Labeled targets on a support synthesized with polymer sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, can be detected by exposing selected regions of sample 1500 to radiation from a source 1100 and detecting the emission therefrom, and repeating the steps of exposition and detection until the sample is completely examined.
87 Citations
90 Claims
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1. An apparatus for imaging a sample located on a support, said apparatus comprising:
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a body for immobilizing said support, said support comprising at least a first surface having said sample thereon;
an electromagnetic radiation source for generating excitation radiation having a first wavelength;
excitation optics for transforming the geometry of said excitation radiation to a line and directing said line at said sample for exciting a plurality of regions thereon, said line causing a labeled material on said sample to emit response radiation, said response radiation having a second wavelength, said first wavelength different from said second wavelength;
collection optics for collecting said response radiation from said plurality of regions;
a detector for sensing said response radiation received by said collection optics, said detector generating a signal proportional to the amount of radiation sensed thereon, said signal representing an image associated with said plurality of regions from said sample;
a translator coupled to said body for allowing a subsequent plurality of regions on said sample to be excited;
a processor for processing and storing said signal so as to generate a 2-dimensional image of said sample; and
a focuser for automatically focusing said sample in a focal plane of said excitation radiation.
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2-65. -65. (canceled)
- 66. A biopolymer array optical scanner system programmed to control power to at least one light source of said system for at least one predetermined time.
- 81. A computer-readable medium comprising a program that controls power to at least one light source of a biopolymer array optical scanner system for at least one predetermined time.
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84. A method of operating a biopolymer array optical scanner system, said method comprising:
- a user selecting and entering into said scanner system at least one predetermined time to control power to at least one light source of the system; and
a software switch controlling power to said at least one light source upon said at least one predetermined time.
- a user selecting and entering into said scanner system at least one predetermined time to control power to at least one light source of the system; and
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90. A kit for use in a biopolymer array optical scanner, said kit comprising:
- a computer-readable medium comprising a program that controls power to at least one light source of a biopolymer array optical scanner system for at least one predetermined time; and
instructions for operating said scanner according to said programming.
- a computer-readable medium comprising a program that controls power to at least one light source of a biopolymer array optical scanner system for at least one predetermined time; and
Specification