Binding assays using molecular melt curves
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Abstract
The present invention provides novel microfluidic devices and methods that are useful for performing binding assays through construction of molecular melt curves. In particular, the devices and methods of the invention are useful in screening large numbers of different test molecules for their binding ability to target molecules.
146 Citations
128 Claims
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1-108. -108. (canceled)
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109. A method of performing binding assays on a plurality of samples, the method comprising:
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(a) loading one of the plurality of samples into a microfluidic device;
(b) directing the one sample to a temperature controlled region of the microfluidic device;
(c) exposing the one sample to a range of temperatures within the temperature controlled region while monitoring a detectable property of the first sample;
(d) directing the one sample out of the temperature controlled region of the microfluidic device; and
repeating steps (a)-(e) for each sample of the plurality of samples. - View Dependent Claims (110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122)
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123. A method of screening a plurality of test compounds to determine whether each of the plurality of test compounds modifies a binding reaction between a first molecule and a second molecule, which binding reaction produces a binding product, the method comprising:
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(a) loading one of the plurality of compounds into a microfluidic device;
(b) mixing the one of plurality of test compounds with a solution comprising the first molecule and the second molecule within a channel in the microfluidic device;
(c) generating a molecular melt curve for the binding product generated within the channel; and
repeating steps (a)-(c) for each sample of the plurality of test compounds. - View Dependent Claims (124, 125, 127)
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126. A method of screening a plurality of test compounds to determine whether each of the plurality of test compounds binds with a first molecule to produce a binding product, the method comprising:
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(a) loading one of the plurality of compounds into a microfluidic device;
(b) mixing the one of plurality of test compounds with a solution comprising the first molecule within a channel in the microfluidic device;
(c) generating a molecular melt curve for the binding product generated within the channel; and
repeating steps (a)-(c) for each sample of the plurality of test compounds.
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128. A method of screening a plurality of test compounds to determine whether each of the plurality of test compounds modifies the thermal stability of a first molecule, the method comprising:
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(a) loading one of the plurality of compounds into a microfluidic device;
(b) mixing the one of plurality of test compounds with a solution comprising the first molecule within a channel in the microfluidic device;
(c) generating a molecular melt curve for the first molecule within the channel; and
repeating steps (a)-(c) for each sample of the plurality of test compounds.
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Specification