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Method and apparatus for amplifying nucleic acids

  • US 20050202489A1
  • Filed: 03/03/2005
  • Published: 09/15/2005
  • Est. Priority Date: 03/12/2004
  • Status: Active Grant
First Claim
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1. A method for amplifying nucleic acids, which comprises:

  • introducing into a reaction vessel via different inlet channels a reactant aqueous solution containing reactants for nucleic acid amplification and a fluid that is phase-separated from the reactant aqueous solution and does not participate in amplification reaction;

    creating a plurality of reactant aqueous solution droplets surrounded by the fluid by contacting the reactant aqueous solution with the fluid in the reaction vessel; and

    amplifying the nucleic acids in the reactant aqueous solution droplets.

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