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Microarray having probe polynucleotide spots capable of binding to the same target polynucleotide fragment maximally separated from each other and a method of producing the same

  • US 20050202492A1
  • Filed: 03/11/2005
  • Published: 09/15/2005
  • Est. Priority Date: 03/12/2004
  • Status: Abandoned Application
First Claim
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1. A polynucleotide microarray having at least two probe polynucleotides capable of binding to the same target polynucleotide immobilized thereon, wherein a spot region on a substrate of the polynucleotide microarray is segmented into blocks arranged in rows and columns adjacent to each other, the number of blocks being 2 m, wherein m is the maximum value of the numbers of the probe polynucleotides capable of binding to the same target polynucleotide, and the spots are arranged such that each block has a width-length ratio of {square root}{square root over (3)}:

  • 1, the blocks are designated as one of block of type a and block of type b so that two blocks of the same type, a or b, do not have sides adjacent to each other, and the probe polynucleotides are immobilized on the block of type a or block of type b so that probe polynucleotides capable of binding to the same target polynucleotide are immobilized at the same corresponding location in selected block of type a or block of type b.

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