Microarray having probe polynucleotide spots capable of binding to the same target polynucleotide fragment maximally separated from each other and a method of producing the same

• US 20050202492A1
• Filed: 03/11/2005
• Published: 09/15/2005
• Est. Priority Date: 03/12/2004
• Status: Abandoned Application

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Shim, Hyeong-sop, Huh, Nam, Oh, Ji-young, Nam, Yun-sun
• Application Number:
  US11/078,601
• Publication Number:
  US 20050202492A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00529 DNA chips
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00693 Means for quality control
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 2565/507 characterised by the density of the capture oligonucleotide
• CPC Class Codes: C12Q 2565/513 characterised by the pattern of the arrayed oligonucleotides

Abstract

The present invention relates to a microarray having probe polynucleotide spots capable of binding to the same target polynucleotide fragment maximally separated from each other, and a method of producing the same.

Claims

1. A polynucleotide microarray having at least two probe polynucleotides capable of binding to the same target polynucleotide immobilized thereon,

wherein a spot region on a substrate of the polynucleotide microarray is segmented into blocks arranged in rows and columns adjacent to each other, the number of blocks being 2 m, wherein m is the maximum value of the numbers of the probe polynucleotides capable of binding to the same target polynucleotide, and the spots are arranged such that each block has a width-length ratio of {square root}{square root over (3)}:

1,

the blocks are designated as one of block of type a and block of type b so that two blocks of the same type, a or b, do not have sides adjacent to each other, and

the probe polynucleotides are immobilized on the block of type a or block of type b so that probe polynucleotides capable of binding to the same target polynucleotide are immobilized at the same corresponding location in selected block of type a or block of type b.

2. The polynucleotide microarray of claim 1, wherein the blocks are separated.

4. The method of claim 2, further comprising forming a space between the blocks.

3. A method of producing a polynucleotide microarray having at least two probe polynucleotides capable of binding to the same target polynucleotide immobilized as spots thereon, comprising:

segmenting a spot region of a substrate of the polynucleotide microarray into blocks arranged in rows and columns adjacent to each other, the number of blocks being 2 m, wherein m is the maximum value of the numbers of the probe polynucleotides capable of binding to the same target polynucleotide, and arranging the spots such that each block has a width-length ratio of {square root}{square root over (3)};

1,

designating the blocks as one of block of type a and block of type b so that two blocks of the same type, a or b, do not have sides adjacent to each other, and

immobilizing the probe polynucleotides on block of type a or block of type b so that probe polynucleotides capable of binding to the same target polynucleotide are immobilized at the same corresponding location in selected block of type a or block of type b