Microarray having probe polynucleotide spots capable of binding to the same target polynucleotide fragment maximally separated from each other and a method of producing the same
First Claim
1. A polynucleotide microarray having at least two probe polynucleotides capable of binding to the same target polynucleotide immobilized thereon, wherein a spot region on a substrate of the polynucleotide microarray is segmented into blocks arranged in rows and columns adjacent to each other, the number of blocks being 2 m, wherein m is the maximum value of the numbers of the probe polynucleotides capable of binding to the same target polynucleotide, and the spots are arranged such that each block has a width-length ratio of {square root}{square root over (3)}:
- 1, the blocks are designated as one of block of type a and block of type b so that two blocks of the same type, a or b, do not have sides adjacent to each other, and the probe polynucleotides are immobilized on the block of type a or block of type b so that probe polynucleotides capable of binding to the same target polynucleotide are immobilized at the same corresponding location in selected block of type a or block of type b.
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Abstract
The present invention relates to a microarray having probe polynucleotide spots capable of binding to the same target polynucleotide fragment maximally separated from each other, and a method of producing the same.
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Citations
4 Claims
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1. A polynucleotide microarray having at least two probe polynucleotides capable of binding to the same target polynucleotide immobilized thereon,
wherein a spot region on a substrate of the polynucleotide microarray is segmented into blocks arranged in rows and columns adjacent to each other, the number of blocks being 2 m, wherein m is the maximum value of the numbers of the probe polynucleotides capable of binding to the same target polynucleotide, and the spots are arranged such that each block has a width-length ratio of {square root}{square root over (3)}: - 1,
the blocks are designated as one of block of type a and block of type b so that two blocks of the same type, a or b, do not have sides adjacent to each other, and the probe polynucleotides are immobilized on the block of type a or block of type b so that probe polynucleotides capable of binding to the same target polynucleotide are immobilized at the same corresponding location in selected block of type a or block of type b. - View Dependent Claims (2, 4)
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3. A method of producing a polynucleotide microarray having at least two probe polynucleotides capable of binding to the same target polynucleotide immobilized as spots thereon, comprising:
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segmenting a spot region of a substrate of the polynucleotide microarray into blocks arranged in rows and columns adjacent to each other, the number of blocks being 2 m, wherein m is the maximum value of the numbers of the probe polynucleotides capable of binding to the same target polynucleotide, and arranging the spots such that each block has a width-length ratio of {square root}{square root over (3)};
1,designating the blocks as one of block of type a and block of type b so that two blocks of the same type, a or b, do not have sides adjacent to each other, and immobilizing the probe polynucleotides on block of type a or block of type b so that probe polynucleotides capable of binding to the same target polynucleotide are immobilized at the same corresponding location in selected block of type a or block of type b
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Specification