Detection of polymorphisms

• US 20050208513A1
• Filed: 10/05/2004
• Published: 09/22/2005
• Est. Priority Date: 04/05/2002
• Status: Abandoned Application

• Current Assignee: ID-Lelystad, Instituut Voor Dierhouderij En Diergezondheid B.V.
• Original Assignee: ID-Lelystad, Instituut Voor Dierhouderij En Diergezondheid B.V.
• Inventors: Agbo, Edwin Chukwura, te Pas, Marinus Frederick Willem, Smits, Marinus Adrianus
• Application Number:
  US10/959,638
• Publication Number:
  US 20050208513A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/683 involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
• CPC Class Codes: C12Q 2525/191 incorporating an adaptor
• CPC Class Codes: C12Q 2535/138 Amplified fragment length polymorphism [AFLP]

Abstract

The present invention provides methods for producing a nucleic acid fingerprint and methods for detecting sequence polymorphisms between one or more genomes. The methods include fragmenting a nucleic acid into nucleic acid fragments with ends that are compatible to ligation with at least one adapter, performing a ligation reaction between the compatible ends of the nucleic acid fragments and at least one adapter, amplifying the nucleic acid fragments by using at least one amplification primer, and generating a nucleic acid fingerprint from the amplified fragments. A method according to the present invention permits high-resolution fingerprinting while maintaining stringency in a PCR reaction. In another aspect, the invention also provides a kit for preparing nucleic acid fingerprints according to methods of the invention.

Claims

1. A process for producing a nucleic acid fingerprint, the process comprising:

fragmenting a starting nucleic acid, thus producing a plurality of adapter ligatable nucleic acid fragments having ends that are compatible to at least one adapter;

performing a ligation reaction between said ends of said plurality of adapter ligatable nucleic acid fragments and the at least one adapter, thus producing adapter-ligated nucleic acid fragments;

amplifying said adapter-ligated nucleic acid fragments with at least one amplification primer that is essentially complementary to a nucleotide sequence of said at least one adapter, thus producing amplified adapter-ligated nucleic acid fragments; and

generating a nucleic acid fingerprint from said amplified adapter-ligated nucleic acid fragments.

2. The process according to claim 1, wherein said plurality of adapter ligatable nucleic acid fragments are obtained by specific fragmentation of said starting nucleic acid.

3. The process according to claim 2, wherein said specific fragmentation comprises digesting said starting nucleic acid with at least two different restriction endonuclease enzymes, thus producing restriction fragments having different ends that are compatible to a single adapter.

4. The process according to claim 2, wherein said specific fragmentation comprises digesting said starting nucleic acid with at least three different restriction endonuclease enzymes, wherein at least two of said at least three different restriction endonuclease enzymes produce restriction fragments having ends that are compatible to a single adapter.

5. The process according to claim 2, wherein said specific fragmentation comprises digesting said starting nucleic acid with at least four different restriction endonuclease enzymes, wherein at least two of said at least four different restriction endonuclease enzymes produce restriction fragments having ends that are compatible to a first adapter.

6. The process according to claim 5, wherein said at least four different restriction endonuclease enzymes comprise at least two enzymes that produce restriction fragments having ends that are compatible to a second adapter.

7. The process according to claim 3, wherein said ends of said restriction fragments comprise cohesive ends.

8. The process according to claim 3, wherein said restriction endonuclease enzymes are 4 to 8 bp-cutting restriction endonuclease enzymes.

9. The process according to claim 3, wherein said restriction endonuclease enzymes that produce compatible ends are selected from the group of enzymes listed in Table 3.

10. The process according to claim 5, wherein said at least four different restriction endonuclease enzymes are selected from the group consisting of:

BglII, BclI, EcoRI and MunI;

BglII, BclI, AcsI and MunI;

BclI, XhoII, AcsI and MunI; and

BglII, XhoII, EcoRI and AcsI.

11. The process according to claim 10, wherein said first adapter is a BglII adapter and said second adapter is a MunI adapter.

12. The process according to claim 1, wherein performing the ligation reaction between said ends of said plurality of adapter ligatable nucleic acid fragments and said at least one adapter comprises forming heterosite nucleic acid fragments.

13. The process according to claim 1, wherein amplifying said adapter-ligated nucleic acid fragments with said at least one amplification primer comprises using at least one primer having selective nucleotides.

14. The process according to claim 1, wherein generating the nucleic acid fingerprint from said amplified adapter-ligated nucleic acid fragments comprises electrophoresing said amplified adapter-ligated nucleic acid fragments.

15. The process according to claim 14, further comprising visualizing said nucleic acid fingerprint with fluorimetry, autoradiography, phospho-imaging, or other methods of visualizing nucleic acid fingerprints.

16. The process according to claim 1, wherein said starting nucleic acid is genomic DNA.

24. A kit for performing the process according to claim 1, comprising an element selected from the group of elements consisting of restriction endonuclease enzymes, buffers, double-stranded DNA adapters, DNA ligase enzymes, cognate amplification primers, DNA polymerase enzymes, reference DNA and data analysis software, and any combination thereof.

25. The kit of claim 24, wherein the cognate amplification primers are labeled with a detectable label.

28. The process according to claim 1, wherein the at least one adapter comprises a first and a second set adapter and the at least one amplification primer comprises two primers having selective nucleotides.

17. A method for detecting sequence polymorphisms between one or more genomes, the method comprising:

producing at least one nucleic acid fingerprint from one or more genomes with a process, the process comprising;

fragmenting the one or more genomes, thus producing a plurality of adapter ligatable nucleic acid fragments having ends that are compatible to at least one adapter;

performing a ligation reaction between said ends of said plurality of adapter ligatable nucleic acid fragments and the at least one adapter thus producing adapter-ligated nucleic acid fragments;

amplifying said adapter-ligated nucleic acid fragments with at least one amplification primer that is essentially complementary to a nucleotide sequence of said at least one adapter, thus producing amplified adapter-ligated nucleic acid fragments; and

generating at least one nucleic acid fingerprint from said amplified adapter-ligated nucleic acid fragments; and

comparing the at least one nucleic acid fingerprint for the presence of, the absence of, or differences between said amplified adapter-ligated nucleic acid fragments to determine the presence of sequence polymorphisms.

18. The method according to claim 17, wherein the one or more genomes are genomes from lower level taxa.

19. The method according to claim 18, wherein said lower level taxa comprises species, subspecies, strains, variatas, forms, cultivars or sub-populations.

20. The method according to claim 17, wherein the one or more genomes are genomes from parasites.

21. A method for the identification of DNA markers linked to a specific phenotype, a phenotypic characteristic, a genetic trait or any combination thereof, the method comprising:

detecting sequence polymorphisms with the method according to claim 17 between said one or more genomes originating from organisms which exhibit differences in the specific phenotype, the phenotypic characteristic, the genetic trait or any combination thereof; and

correlating said polymorphisms to said differences.

22. The method according to claim 21, wherein said DNA markers are selected from the group consisting of:

SNP markers, multi-locus markers, single locus markers, dominant markers, diagnostic markers, phenotypic markers, clonal markers, reference markers, and any combination thereof.

23. A method for identifying an unknown organism, the method comprising:

producing a nucleic acid fingerprint from said unknown organism with a process, the process comprising;

fragmenting a starting nucleic acid thus producing a plurality of adapter ligatable nucleic acid fragments having ends that are compatible to at least one adapter;

performing a ligation reaction between said ends of said plurality of adapter ligatable nucleic acid fragments and the at least one adapter, thus producing adapter-ligated nucleic acid fragments;

amplifying said adapter-ligated nucleic acid fragments with at least one amplification primer that is essentially complementary to a nucleotide sequence of said at least one adapter, thus producing amplified adapter-ligated nucleic acid fragments; and

generating a nucleic acid fingerprint from said amplified adapter-ligated nucleic acid fragments;

comparing said nucleic acid fingerprint with at least one known nucleic acid fingerprint produced from at least one known organism with said process; and

establishing an identity of said unknown organism on the basis of similarity of said nucleic acid fingerprint from said unknown organism with said at least one known nucleic acid fingerprint from said at least one known organism.

26. A kit comprising:

at least one set of at least two different restriction endonuclease enzymes capable of producing restriction fragments having ends that are compatible with a single type of adapter; and

at least said one single type of adapter.

27. The kit of claim 26, further comprising at least one primer that is essentially complementary to said adapter.