Dinoflagellate karlotoxins, methods of isolation and uses thereof

• US 20050209104A1
• Filed: 08/19/2003
• Published: 09/22/2005
• Est. Priority Date: 08/19/2002
• Status: Active Application

• Current Assignee: University of Maryland Biotechnology Institute
• Original Assignee: University of Maryland Biotechnology Institute
• Inventors: Place, Allen, Deeds, Jonathan R.
• Application Number:
  US10/525,711
• Publication Number:
  US 20050209104A1
• Time in Patent Office: Days
• US Class Current: 504/150
• CPC Class Codes: C07K 14/00 Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
• CPC Class Codes: C07K 14/44 from protozoa

Abstract

Disclosed are six dinoflagellate toxins isolated from Karlodinium micrum and methods of isolating said toxins. The toxins are useful in killing cells, such as in the killing of tumor cells in an animal. Antibodies against these toxins allow detection of the toxins and the dinoflagellate as well as a method of neutralizing the toxin.

Claims

1. An isolated toxin from Karlodinium micrum.

2. The isolated toxin according to claim 1 comprising a member selected from the group consisting of KmTx 1, KmTx 2, KmTx 3, KmTx 4, KmTx 5 and KmTx 6.

3. The isolated toxin according to claim 1 comprising KmTx 1, wherein the KmTx 1 toxin is eluted at about 22-24 minutes of reversed phase HPLC elution of Karlodinium micrum.

4. The isolated toxin according to claim 1 comprising KmTx 3, wherein the KmTx 3 toxin is eluted at about 16-18 minutes of reversed phase HPLC elution of Karlodinium micrum.

5. The isolated toxin according to claim 1 comprising KmTx 1, wherein the KmTx 1 toxin is eluted at about 22 to 24 minutes of reversed phase HPLC fractions of a concentrated 80% MeOH tC₁₈ elution of Karlodinium micrum.

6. The isolated toxin according to claim 1 comprising KmTx 3, wherein the KmTx 3 toxin is eluted at about 16-18 minutes of reversed phase HPLC fractions of a concentrated 80% MeOH tC₁₈ elution of Karlodinium micrum.

7. The isolated toxin according to claim 1 comprising a molecular mass of 1362 daltons.

8. The isolated toxin according to claim 1 comprising a molecular mass of 1344 daltons.

9. A method of producing a karlotoxin comprising the steps of:

a) culturing Karlodinium micrum in a medium suitable for production of toxin; and

b) isolating the toxin.

10. The method according to claim 9 comprising a member selected from the group consisting of KmTx 1, KmTx 2, KmTx 3, KmTx 4, KmTx 5 and KmTx 6.

11. The method according to claim 9, wherein the toxin is isolated by separation on a HPLC column.

12-19. -19. (canceled)

20. A method of inhibiting a Karlodinium micrum toxin comprising contacting an antibody which specifically binds said toxin.

21. The method of claim 20, wherein the toxin is the toxin comprises a member selected from the group consisting of KmTx 1, KmTx 2, KmTx 3, KmTx 4, KmTx 5 and KmTx 6.

22. The method of claim 20, wherein the toxin is the toxin comprises KmTx 1 and is eluted at about 22-24 minutes of reversed phase HPLC elution of Karlodinium micrum.

23. The method of claim 20, wherein the toxin is the toxin comprises KmTx 3 and is eluted at about 17-18 minutes of reversed phase HPLC elution of Karlodinium micrum.

24. An immunoconjugate comprising a Karlodinium micrum toxin linked to an antibody.

25. The immunoconjugate of claim 24, wherein the toxin comprises a member selected from the group consisting of KmTx 1, KmTx 2, KmTx 3, KmTx 4, KmTx 5 and KmTx 6.

26. The immunoconjugate of claim 25, wherein the antibody is an anti-tumor antibody.

27-28. -28. (canceled)

29. A composition comprising a Karlodinium micrum toxin.

30. The composition according to claim 29, wherein the toxin comprises a member selected from the group consisting of KmTx 1, KmTx 2, KmTx 3, KmTx 4, KmTx 5 and KmTx 6.

31. The composition according to claim 29, wherein the toxin comprises KmTx 1 and is eluted at about 22-24 minutes of reversed phase HPLC elution of Karlodinium micrum.

32. The composition according to claim 29, wherein the toxin comprises KmTx 3 and is eluted at about 17-18 minutes of reversed phase HPLC elution of Karlodinium micrum.

33. A method of treating blooms in an aqueous medium caused by K. micrum to reduce mortality rate of fish exposed to the treatment, the method comprising:

introducing an algicidal composition in an effective amount to reduce the level of K. micrum in the aqueous medium, wherein the algicidal composition comprises potassium permanganate and does not include copper sulfate.

34-36. -36. (canceled)