Pluripotent mammalian cells

• US 20050210537A1
• Filed: 05/18/2005
• Published: 09/22/2005
• Est. Priority Date: 06/15/2000
• Status: Abandoned Application

• Current Assignee: Alan Colman, Raymond L. Page, Tanja Dominko, Todd Vaught, Vivienne Marshall
• Original Assignee: Alan Colman, Raymond L. Page, Tanja Dominko, Todd Vaught, Vivienne Marshall
• Inventors: Colman, Alan, Vaught, Todd, Marshall, Vivienne, Dominko, Tanja, Page, Raymond L.
• Application Number:
  US11/131,703
• Publication Number:
  US 20050210537A1
• Time in Patent Office: Days
• US Class Current: 800/14
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C12N 15/873 Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
• CPC Class Codes: C12N 2506/04 from germ cells
• CPC Class Codes: C12N 2517/04 Cells produced using nuclear transfer
• CPC Class Codes: C12N 2517/10 Conditioning of cells for in vitro fecondation or nuclear transfer
• CPC Class Codes: C12N 5/16 Animal cells

Abstract

The invention relates to a method of making pluripotent stem cells that does not involve the formation of early preimplantation embryos or fetal tissue. The method has general utility in the production of pluripotent stem cells from many mammalian species but has particular application in man where pluripotent stem cell production can be customized to particular human individual. The method involves the fusion of donor somatic or stem cells (or their karyoplasts) with cytoplasmic, membrane-delimited fragments of mammalian oocytes or zygotes. After the initial genomic reprogramming occurs, the cells can proliferate and thus multiply in vitro yielding a large number of autologous cells for cell therapy application. The result of this process is a cell population genomically identical to the somatic, differentiated cells derived from an individual patient. However, these cells are pluripotent in that upon application of specific growth factors, the cells are capable of differentiating into specific cell types as required by the sought clinical indication.

Claims

1-47. -47. (canceled)

48. A method of generating a hybrid mammalian cell comprising:

(a) preparing more than one cytoplast fragment from a mammalian metaphase 11 oocyte or fertilized zygote wherein the amount of cytoplasm in the cytoplast fragment is less than the amount of cytoplasm in the mammalian oocyte or fertilized zygote;

(b) obtaining a nuclear donor cell or karyoplast taken from a mammal;

(c) combining one cytoplast fragment of step a) with the nuclear donor cell or karyoplast of step b) to produce a hybrid mammalian cell; and

(d) if an oocyte is used in step (a), then activating the oocyte before, during or after step (c).

49. The method of claim 48, wherein the cytoplast fragment is produced by vortexing the mammalian oocyte or fertilized zygote.

50. The method of claim 48, wherein the mammalian oocyte or fertilized zygote is surrounded by a zona pellucida and wherein the zona pellucida is removed prior to step (a).

51. The method of claim 48, wherein the mammalian oocyte-fertilized zygote, or resulting fragment thereof is enucleated.

52. The method of claim 48, wherein the mammalian oocyte is matured in vitro or in vivo.

53. The method of claim 48, wherein the mammalian oocyte is selected from the group consisting of:

an activated, low maturation promotion factor oocyte;

an aged, unactivated, low maturation promotion factor oocyte; and

an unactivated, high maturation promotion factor, metaphase II oocyte.

54. The method of claim 48, wherein the cytoplast fragment is from a different species from that of the nuclear donor.

55. The method of claim 48, wherein the cytoplast fragment is from the same species as that of the nuclear donor.

56. The method of claim 48, wherein the cytoplast fragment is prepared from a mammalian oocyte or fertilized zygote taken from a non-human mammalian species.

57. The method of claim 48, wherein the nuclear donor cell is selected from the group consisting of fibroblasts, skin fibroblasts, leukocytes, granulosa cells, cumulus cells, oviductal epithelium, mammary gland cells, fetal fibroblasts, keratinocytes, hepatocytes, respiratory epithelial cells, neuronal cells, CD34+stem cells, granulocytes, and mononuclear peripheral blood cells.

58. The method of claim 48, further comprising maintaining the pluipotency by placing the cell in a culture media that supports development and proliferation while maintaining the dedifferentiated state.

59. The method of claim 48, wherein the combining of the cytoplast fragment with the nuclear donor is mediated by electrical fusion, chemical fusion, viruses, liposomes or cell surface proteins.

60. The method of claim 48, wherein the nuclear donor is from an embryonic, fetal, or adult cell, or an embryonic, fetal, or adult karyoplast.

61. The method of claim 48, wherein the nuclear donor is a diploid cell or is taken from a diploid cell.

62. The method of claim 48, wherein the nuclear donor is from a stem cell, or differentiated or undifferentiated somatic cell.

63. The method of claim 48, wherein the nuclear donor has been genetically modified.

64. The method of claim 48, further comprising the step of establishing a population of hybrid cells derived from the hybrid cell.

65. The method of claim 48, wherein more than 10 cytoplast fragments are prepared.

66. A method for reprogramming mammalian cells comprising:

(a) preparing more than one cytoplast fragment from a mammalian mammalian metaphase II oocyte or fertilized zygote wherein the amount of cytoplasm in the cytoplast fragment is less than the amount of cytoplasm in the mammalian oocyte or fertilized zygote;

(b) obtaining a nuclear donor cell or karyoplast taken from a mammal; and

(c) combining one cytoplast fragment of step a) with the nuclear donor cell or karyoplast of step b) to produce a reprogrammed mammalian cell; and

(d) if an oocyte is used in step (a), then activating the oocyte before, during or after step (c).

67. The method of claim 66, wherein the reprogrammed mammalian cell is a cardiomycyte.

68. A method of generating a hybrid mammalian cell comprising:

(a) preparing more than one cytoplast fragment from a mammalian metaphase II oocyte or fertilized zygote wherein the amount of cytoplasm in the cytoplast fragment is less than the amount of cytoplasm in the mammalian oocyte or fertilized zygote;

(b) obtaining nuclear donor cell or karyoplast taken from a mammal; and

(c) combining one cytoplast fragment of step a) with the nuclear donor cell or karyoplast of step (b) to produce a hybrid mammalian cell; and

(d) if an oocyte is used in step (a), then activating the oocyte before, during or after step (c).