Method for multiple sample screening using IR spectroscopy

• US 20050211555A1
• Filed: 01/18/2005
• Published: 09/29/2005
• Est. Priority Date: 02/14/2002
• Status: Abandoned Application

• Current Assignee: Solus Biosystems, Inc.
• Original Assignee: Solus Biosystems, Inc.
• Inventors: Archibald, William B.
• Application Number:
  US11/038,550
• Publication Number:
  US 20050211555A1
• Time in Patent Office: Days
• US Class Current: 204/452
• CPC Class Codes: B01J 2219/00313 the reactor vessels being formed by arrays of wells in blocks
• CPC Class Codes: B01J 2219/00702 Processes involving means for analysing and characterising the products
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 2300/0851 Bottom walls
• CPC Class Codes: B01L 2300/12 Specific details about materials
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence
• CPC Class Codes: G01N 21/21 Polarisation-affecting properties G01N21/19 takes precedence
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/33 using ultraviolet light G01N21/39 takes precedence
• CPC Class Codes: G01N 21/3563 for analysing solids; Preparation of samples therefor
• CPC Class Codes: G01N 21/3577 for analysing liquids, e.g. polluted water
• CPC Class Codes: G01N 21/3581 using far infrared light; using Terahertz radiation
• CPC Class Codes: G01N 21/359 using near infrared light
• CPC Class Codes: G01N 21/552 Attenuated total reflection
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 21/7703 : using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B

Abstract

A method for analyzing a fluid sample is provided which includes providing a plurality of fluid samples to be analyzed and inputting each one of the plurality of fluid samples into corresponding ones of a plurality of capillaries defined within a substrate. The method further includes applying a positive charge to each one of the plurality of fluid samples at a first end of each one of the plurality of capillaries and a negative charge to each one of the plurality of fluid samples at a second end of each one of the plurality of capillaries. The method also includes transmitting an infrared light through each one of the plurality of fluid samples at a substantially same time and detecting the infrared light transmitted through each one of the plurality of fluid samples. The method further includes generating an absorption map capable of being displayed as at least one data point based on the detection of the infrared light transmitted through each one of the plurality of fluid samples.

Claims

1. A method for analyzing fluid samples, comprising:

providing a plurality of fluid samples to be analyzed;

inputting each one of the plurality of fluid samples into corresponding ones of a plurality of capillaries defined within a substrate;

applying a positive charge to each one of the plurality of fluid samples at a first end of each one of the plurality of capillaries and a negative charge to each one of the plurality of fluid samples at a second end of each one of the plurality of capillaries;

transmitting an infrared light through each one of the plurality of fluid samples at a substantially same time;

detecting the infrared light transmitted through each one of the plurality of fluid samples; and

generating an absorption map capable of being displayed as at least one data point based on the detection of the infrared light transmitted through each one of the plurality of fluid samples.

2. A method for analyzing fluid samples as recited in claim 1, wherein generating an absorption map capable of being displayed as at least one data point includes generating at least one absorption spectrum of infrared light for each one of the plurality of fluid samples.

3. A method for analyzing fluid samples as recited in claim 1, further comprising:

characterizing each one of the plurality of fluid samples based on the at least one absorption spectrum of infrared light.

4. A method for analyzing fluid samples as recited in claim 1, wherein applying a positive charge to each one of the plurality of fluid samples at a first end of each one of the plurality of capillaries and a negative charge to each one of the plurality of fluid samples at a second end of each one of the plurality of capillaries includes generating a pH gradient between the first end and the second end of each one of the plurality of capillaries.

5. A method for analyzing fluid samples as recited in claim 1, wherein the fluid samples are biological samples.

6. A method for analyzing fluid samples as recited in claim 1, wherein the infrared light is generated by an interferometer.

7. A method for analyzing fluid samples as recited in claim 1, wherein each one of the plurality of capillaries are coated with a material to reduce surface charge.

8. A method for analyzing a fluid sample, comprising:

providing a fluid sample to be analyzed;

inputting the fluid sample into a capillary defined within a substrate;

applying a positive charge to the fluid sample at a first end of the capillary and a negative charge to the fluid sample at a second end of the capillary;

transmitting an infrared light through the fluid sample

detecting the infrared light transmitted through fluid sample; and

generating an absorption map capable of being displayed as at least one data point based on the detection of the infrared light transmitted through the fluid sample.

9. A method for analyzing a fluid sample as recited in claim 8, wherein generating an absorption map capable of being displayed as at least one data point includes generating at least one absorption spectrum of infrared light for the fluid sample.

10. A method for analyzing a fluid sample as recited in claim 8, further comprising:

characterizing the fluid sample based on the at least one absorption spectrum of infrared light.

11. A method for analyzing a fluid sample as recited in claim 8, wherein applying a positive charge to the fluid sample at a first end of the capillary and a negative charge to the fluid sample at the second end of the capillary includes generating a pH gradient between the first end and the second end of the capillary.

12. A method for analyzing a fluid sample as recited in claim 8, wherein the fluid sample is a biological sample.

13. A method for analyzing a fluid sample as recited in claim 8, wherein the infrared light is generated by an interferometer.

14. A method for analyzing fluid samples as recited in claim 8, wherein the fluid samples are biological samples.

15. A computer readable media with program instructions for analyzing a fluid sample, the computer readable media comprising:

program instructions for providing a fluid sample to be analyzed;

program instructions for inputting the fluid sample into a capillary defined within a substrate;

program instructions for applying a positive charge to the fluid sample at a first end of the capillary and a negative charge to the fluid sample at a second end of the capillary;

program instructions for transmitting an infrared light through the fluid sample;

program instructions for detecting the infrared light transmitted through fluid sample; and

program instructions for generating an absorption map capable of being displayed as at least one data point based on the detection of the infrared light transmitted through the fluid sample.

16. A computer readable media as recited in claim 15, wherein the program instructions for generating an absorption map capable of being displayed as at least one data point includes program instructions for generating at least one absorption spectrum of infrared light for the fluid sample.

17. A computer readable media as recited in claim 15, further comprising:

program instructions for characterizing the fluid sample based on the at least one absorption spectrum of infrared light.

18. A computer readable media as recited in claim 15, wherein program instructions for applying a positive charge to the fluid sample at a first end of the capillary and a negative charge to the fluid sample at the second end of the capillary includes program instructions for generating a pH gradient between the first end and the second end of the capillary.

19. A computer readable media as recited in claim 15, wherein the fluid sample is a biological sample.

20. A computer readable media as recited in claim 15, wherein the infrared light is generated by an interferometer.

21. A computer readable media as recited in claim 15, wherein the fluid samples are biological samples.