Electrochemical sensor and method for continuous analyte monitoring
First Claim
1. A method for monitoring analyte concentration in a biological fluid by use of a sensor, the method comprising the steps of:
- providing a sensor comprising a volume of a hydrophilic medium for retaining an amount of analyte proportionate to the concentration of analyte in a biological fluid in contact with the sensor, an enclosure for said analyte retention volume, an electrode in contact with the hydrophilic medium, a redox enzyme in contact with the medium, and an electron transfer mediator for facilitating transfer of electrons between the enzyme and the electrode, the enzyme substantially not capable of transferring electrons to or from components of the biological fluid other than said analyte, contacting the biological fluid with the sensor and at initially predetermined intervals intermittently applying a potential to the electrode sufficient to oxidize the electron mediator and sensing current through said electrode as a function of the duration of the applied potential, maintaining the applied mediator oxidizing applied potential at least for a period of time sufficient to determine the rate of change of current with time through the electrode, and correlating the current flow with the current flow for known concentrations of said analyte in the retention medium.
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Abstract
Sensors and a method for detecting an analyte are described. Sensors each have a volume of a hydrophilic medium that retains an amount of analyte proportionate to the concentration of analyte in a biological fluid, electrodes and a redox enzyme in contact with medium, and an electron transfer mediator. The fluid contacts sensors and at initially predetermined intervals intermittently applies a potential to electrode sufficient to oxidize the mediator and sensing current through electrode as a function of the duration of the applied potential. The applied mediator oxidizing applied potential is maintained for a period of time sufficient to determine the rate of change of current with time through electrode. The current flow is correlated with the current flow for known concentrations of the analyte in medium.
183 Citations
6 Claims
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1. A method for monitoring analyte concentration in a biological fluid by use of a sensor, the method comprising the steps of:
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providing a sensor comprising a volume of a hydrophilic medium for retaining an amount of analyte proportionate to the concentration of analyte in a biological fluid in contact with the sensor, an enclosure for said analyte retention volume, an electrode in contact with the hydrophilic medium, a redox enzyme in contact with the medium, and an electron transfer mediator for facilitating transfer of electrons between the enzyme and the electrode, the enzyme substantially not capable of transferring electrons to or from components of the biological fluid other than said analyte, contacting the biological fluid with the sensor and at initially predetermined intervals intermittently applying a potential to the electrode sufficient to oxidize the electron mediator and sensing current through said electrode as a function of the duration of the applied potential, maintaining the applied mediator oxidizing applied potential at least for a period of time sufficient to determine the rate of change of current with time through the electrode, and correlating the current flow with the current flow for known concentrations of said analyte in the retention medium. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification