Multidimensional pump apparatus and method for fully automated complex mixtures separation, identification, and quantification
First Claim
1. A fluid pump containing an optical encoder to facilitate digital closed loop control for synchronizing piston refill begin and end strokes with the opening and closing of electrically actuated or pneumatically actuated valve or valves switching.
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Abstract
A fluid pumping system can be operated in dual control modes: as a syringe pump for nano-flow solvent delivery; and as a reciprocating pump for micro- and analytical flow solvent delivery. The fluid pumping system is also operated in a closed-loop digital control process using an optical encoder for piston refill stroke begin and end synchronization with a switching valve On and Off. A multidimensional apparatus and procedure using up to 8 of the invented fluid pumps for a fully automated procedure such as ICA™ chemistry for cellular protein separation, identification and quantification.
19 Citations
10 Claims
- 1. A fluid pump containing an optical encoder to facilitate digital closed loop control for synchronizing piston refill begin and end strokes with the opening and closing of electrically actuated or pneumatically actuated valve or valves switching.
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6. A method of proportioning fluid to form a fluid composition or flow gradient, comprising the steps of:
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Providing a plurality of pumps each connected to a respective fluid from a corresponding one of a plurality of reservoirs or each connected to a plurality of reservoirs;
Providing a plurality of switching valves each connected to a column or columns for on-line fluidic switching;
Providing a plurality of separation columns to facilitate a separation of complex sample or samples based on a combination of more than two of the following separation methods;
reversed phase, normal phase, ion exchange, size exclusion, affinity chromatography, gel permeation chromatography, electrical field assisted PH gradient chiral chromatography, metal chelating or affinity, isotope labeling tag, super-critical fluid chromatography, high temperature liquid chromatography, capillary electrophoresis(CE), capillary electro-chromatography(CEC), displacement chromatography, perfusion chromatography, and turbulence flow chromatography;
A gradient proportioning pumping system for on-line fully automated separation of proteins or the reaction products of proteins. An example based on the Isotope-Coded Affinity Tag (ICAT™
) chemistry as presented by Goodlett et al (U.S. Pat. No.;
6,629,040), or based on IMAC (Immobilized metal affinity chromatography (IMAC) (L. Anderson et al Anal biochem. 1986, 154(10), 250-254), comprising;
A multi-channel pumping system including Six to eight pumps for a plurality of solvents;
a strong cation exchange column, a reversed phase trapping column, an affinity column (Avidin affinity column), a cleavage column or multiple of cleavage columns, a nano-trap column, an analysis nano-LC column, a Mass Spectrometer, an auto-sampler, an injection valve or switching valve, three to four additional flow path diverting valves, one to two Tees, a spray tip or a nano-column with spray tip for Mass Spectrometer, and the Reagents prescribed in the Applied Biosystems ICAT™
kit (Applied Biosystems ICAT™
Cleavable ICAT™
Reagent Methods Development Kit, Part number
4339035). - View Dependent Claims (7, 8, 10)
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Specification