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Detection of nucleic acid hybrids

  • US 20050214753A1
  • Filed: 02/22/2001
  • Published: 09/29/2005
  • Est. Priority Date: 03/13/1998
  • Status: Abandoned Application
First Claim
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1. A method for determining the presence or absence of a predetermined nucleic acid target sequence in a nucleic acid sample that comprises the steps of:

  • (A) providing a treated sample that may contain said predetermined nucleic acid target sequence hybridized with a nucleic acid probe that includes an identifier nucleotide in the 3′

    -terminal region;

    (B) admixing the treated sample with a depolymerizing amount of an enzyme whose activity is to release one or more nucleotides from the 3′

    -terminus of a hybridized nucleic acid probe to form a treated reaction mixture;

    (C) maintaining the treated reaction mixture for a time period sufficient to permit the enzyme to depolymerize hybridized nucleic acid and release identifier nucleotides therefrom; and

    (D) analyzing for the presence of released identifier nucleotides to obtain an analytical output, the analytical output indicating the presence or absence of said nucleic acid target sequence.

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