Novel elongase gene and method for producing polyunsaturated fatty acids
First Claim
1. An isolated nucleic acid encoding a polypeptide that elongates C16- or C18fatty acids with at least two double bonds in the fatty acid by at least two carbon atoms, wherein one or more of C18.3Δ
- 5t,9,12, C20;
3Δ
8,11,14, C20;
4Δ
5,8,11,14 and C20;
50Δ
5,8,11,14,17 are not elongated.
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Abstract
The invention relates to a novel elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives or analogs, to a gene construct comprising this gene or its homologs, derivatives and analogs, and to its use. The invention also relates to vectors or organisms comprising an elongase gene with the sequence SEQ ID NO:1 or its homologs, derivatives or analogs. Furthermore, the invention relates to a process for the preparation of polyunsaturated fatty acids and to a process for introducing DNA into organisms which produce large amounts of oils and, in particular, oils with a high content of unsaturated fatty acids. Moreover, the invention relates to an oil and/or a fatty acid preparation with a higher content of polyunsaturated fatty acids with at least two double bonds and/or a triacylglycerol preparation with a higher content of polyunsaturated fatty acids with at least two double bonds.
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Citations
25 Claims
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1. An isolated nucleic acid encoding a polypeptide that elongates C16- or C18fatty acids with at least two double bonds in the fatty acid by at least two carbon atoms, wherein one or more of C18.3Δ
- 5t,9,12, C20;
3Δ
8,11,14, C20;
4Δ
5,8,11,14 and C20;
50Δ
5,8,11,14,17 are not elongated. - View Dependent Claims (5, 6, 7, 8, 22, 23, 24, 25)
- 5t,9,12, C20;
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2. An isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide that elongates C16- or C18-fatty acids with at least two double bonds in the fatty acid molecule, wherein said nucleotide sequence is selected from the group consisting of:
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a) the sequence of SEQ ID NO;
1;
b) a nucleic acid sequence which, in accordance with degeneracy of the genetic code, is derived from the amino acid sequence of SEQ ID NO;
2; and
c) derivatives of the sequence of SEQ ID NO;
1 which encode polypeptides with at least 50% homology with the amino acid sequences of SEQ ID NO;
2, wherein the sequence acts as a C16- or C18-elongase. - View Dependent Claims (3, 4)
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9. An organism comprising a recombinant nucleic acid that encodes a polypeptide, which elongates C16- or C18-fatty acids with at least two double bonds in the fatty acid by at least two carbon atoms, wherein one or more of C18:
- 3Δ
5t,9,12, C20;
3Δ
8,11,14, C20;
4Δ
5,8,11,14 and C20;
5Δ
5,8,14,17 are not elongated. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16, 17, 18)
- 3Δ
- 19. An oil, lipid or fatty acid composition which comprises PUFAs and is derived from a transgenic plant.
Specification