Very large scale immobilized polymer synthesis
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Abstract
A method and apparatus for preparation of a substrate containing a plurality of sequences. Photoremovable groups are attached to a surface of a substrate. Selected regions of the substrate are exposed to light so as to activate the selected areas. A monomer, also containing a photoremovable group, is provided to the substrate to bind at the selected areas. The process is repeated using a variety of monomers such as amino acids until sequences of a desired length are obtained. Detection methods and apparatus are also disclosed.
105 Citations
127 Claims
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1-104. -104. (canceled)
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105. A method for forming a polymer array, the method comprising:
- providing a substrate suitable for synthesizing polymer sequences;
forming an array, each one of a plurality of locations of the array having at least one strand end;
forming photosensitive protection on the strand ends;
selectively scanning and modulating at least one energy beam to expose a pattern on the photosensitive protection;
removing a protective group from selected strand ends based on the exposed pattern; and
adding a predetermined one or more polymeric subunits to the deprotected strand ends. - View Dependent Claims (106, 107, 108, 109, 110)
- providing a substrate suitable for synthesizing polymer sequences;
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111. A system for forming a polymer array on a substrate, the system comprising:
- an energy-beam source that produces an energy beam;
a scanner configured to move the energy beam in a scanned pattern on the substrate;
a computer operatively coupled to the scanner to control the scanned pattern of energy according to a sequence definition for each of a plurality of desired polymer sequences being synthesized;
a reagent source configured to add one of a plurality of monomers at each end location of each of a plurality of strands formed on the substrate; and
apparatus to provide photosensitive protection that controls an order of monomers based on the scanned pattern of light. - View Dependent Claims (112, 113, 114, 115, 116, 117)
- an energy-beam source that produces an energy beam;
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118. A system for forming a polymer array on a substrate, the system comprising:
- means for scanning a pattern of energy on the substrate according to a sequence definition for each of a plurality of desired polymer sequences being synthesized on the substrate;
a reagent source configured to add one of a plurality of monomers at each end location of each of a plurality of strands formed on the substrate; and
means for providing photosensitive protection that controls an order of monomers based on the scanned pattern of light. - View Dependent Claims (119, 120, 121, 122, 123)
- means for scanning a pattern of energy on the substrate according to a sequence definition for each of a plurality of desired polymer sequences being synthesized on the substrate;
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124. A system for forming a polymer microarray on a substrate, the system comprising:
- a first ultraviolet laser;
a first scanner configured to move light from the first ultraviolet laser in a scanned pattern on the substrate;
a computer operatively coupled to the first scanner to control the scanned pattern of light according to a sequence definition for each of a plurality of desired polymer sequences being synthesized;
a reagent source configured to add one of a plurality of monomers at each end location of each of a plurality of strands formed on the substrate; and
apparatus to provide photoresist layer that is patterned by the scanned light pattern to controls an order of monomers based on the computer-controlled scanned pattern of light. - View Dependent Claims (125, 126, 127)
- a first ultraviolet laser;
Specification