Self-contained microfluidic biochip and apparatus

• US 20050221281A1
• Filed: 04/29/2005
• Published: 10/06/2005
• Est. Priority Date: 01/08/2003
• Status: Abandoned Application

• Current Assignee: Winston Z. Ho
• Original Assignee: Winston Z. Ho
• Inventors: Ho, Winston Z.
• Application Number:
  US11/117,908
• Publication Number:
  US 20050221281A1
• Time in Patent Office: Days
• US Class Current: 435/4
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/049 vacuum
• CPC Class Codes: B01L 2400/0683 mechanically breaking a wall or membrane within a channel or chamber
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/523 with means for closing or opening
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

A biochip and apparatus is disclosed for performing biological assays in a self-contained microfluidic platform. The disposable biochip for multi-step reactions comprises a body structure with a plurality of reagent cavities and reaction wells connected via microfluidic channels; the reagent cavities with reagent sealing means for storing a plurality of reagents; the reagent sealing means being breakable and allowing a sequence of reagents to be released into microfluidic channel and reaction well; and the reaction well allowing multi-step reactions to occur. The apparatus may further comprise a microactuator, a heating and cooling element, a detector, a moving stage, a magnetic field generator, and a processor operable to perform all necessary functions, such as reagent delivery, magnetic purification, mixing and incubation, heating and cooling, and optical detection on a microfluidic biochip.

Claims

12. A method for measuring optical properties of a fluid combining at least two reagents in a biochip system, the method comprising:

providing the biochip system having a biochip, a releasing actuator, a detector, a moving stage, and a processor, wherein said biochip comprises a plurality of patterned reagent wells with self-contained reagents that are connected to reaction wells via microchannels, the moving stage being mounted at the biochip, the moving stage being sized and configured to accurately position each of said reagent wells under said actuator;

releasing a first self-contained reagent from a first reagent well by positioning and activating the releasing actuator onto a first reagent well and transporting released first reagent to a first reaction well;

releasing a second self-contained reagent from a second reagent well by positioning and activating the releasing actuator onto a second reagent well and transporting released second reagent to the first reaction well;

moving the first reaction well to proximity of the detector using the moving stage;

measuring optical properties of fluid in said first reaction well using the detector; and

processing the optical properties of the fluid using the processor.

1. An analytical apparatus for use with a microfluidic biochip that comprises a plurality of patterned reagent wells with self-contained reagents that are connected to reaction wells via microchannels, the apparatus comprising:

(a) a micro mechanical actuator for delivering downward pressure to transport at least one of said reagents from said patterned reagent wells into a first reaction well;

(b) a detector for measuring optical properties of fluid in said first reaction well;

(c) a moving stage mounted at the biochip, wherein the moving stage is sized and configured to accurately position each of said reagent wells under said actuator, and position said first reaction well above or below said detector; and

(d) a processor configured to control said moving stage and process the optical properties.

2. The apparatus of claim 1, further comprising a vacuum suction connected to said first reaction well and remove the fluid away from said first reaction well.

3. The apparatus of claim 1, further comprising a magnetic field generator situated in proximity of said first reaction well, wherein the generator has on and off switching mechanisms.

4. The apparatus of claim 3, wherein said magnetic field generator is an external magnet or a built-in electromagnetic element.

5. The apparatus of claim 1, wherein at least one of self-contained reagents comprises magnetic particles.

6. The apparatus of claim 1, wherein said moving stage is an X-Y translation stage or a rotational stage.

7. The apparatus of claim 1, further comprising a heating and cooling element for controlling a temperature of the fluid.

8. The apparatus of claim 7, wherein the moving stage is further configured to accurately position the first reaction well above said heating and cooling element.

9. The apparatus of claim 7, wherein said heating and cooling element is a Peltier thermal heat pump.

10. The apparatus of claim 1, wherein said optical properties are chemiluminescence or bioluminescence signals.

11. The apparatus of claim 1, further comprising a light source located above or below the biochip, wherein the light source illuminates said first reaction well for light absorbance or fluorescence measurement.

13. The method of claim 12, wherein the biochip system further comprises a vacuum suction connected to said first reaction well, said vacuum suction removing the fluid away from said first reaction well.

14. The method of claim 12, wherein the biochip system further comprises a magnetic field generator situated in proximity of said first reaction well, wherein said magnetic field generator has on and off switching mechanisms, and wherein said magnetic field generator is an external magnet or a built-in electromagnetic element.

15. The method of claim 12, wherein at least one of self-contained reagents comprises magnetic particles.

16. The method of claim 12, wherein said moving stage is an X-Y translation stage or a rotational stage.

17. The method of claim 12, wherein the biochip system further comprises a heating and cooling element for controlling a temperature of the fluid.

18. The method of claim 17, wherein the moving stage is further configured to accurately position the first reaction well above said heating and cooling element.

19. The method of claim 12, wherein said optical properties are chemiluminescence or bioluminescence signals.

20. The method of claim 12, wherein the biochip system further comprises a light source for illuminating said first reaction well for light absorbance or fluorescence measurement.

18-1. The method of claim 17, wherein said heating and cooling element is a Peltier thermal heat pump.