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Methods of detecting target molecules and molecular interactions

  • US 20050221349A1
  • Filed: 11/29/2004
  • Published: 10/06/2005
  • Est. Priority Date: 05/30/2002
  • Status: Abandoned Application
First Claim
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1. A method of detecting a target molecule in a sample comprising the steps of;

  • (a) contacting a sample with two or more binding entities specific for the target molecule, including a first binding entity labelled with a catalytic label and a second, separate binding entity labelled with a substrate label, characterised in that the catalytic label is capable of acting directly on the substrate label to generate a detectable change in the substrate label;

    (b) incubating under conditions which permit the binding entities to bind to the target molecule, thus bringing the catalytic and substrate labels into close proximity;

    (c) allowing the catalytic label to act directly on the substrate label when bound in close proximity, thereby producing a detectable change in the substrate label;

    (d) detecting the change in the substrate label.

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