Methods and devices for microarray image analysis

• US 20050221351A1
• Filed: 12/30/2004
• Published: 10/06/2005
• Est. Priority Date: 04/06/2004
• Status: Abandoned Application

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Ryu, Jekwam
• Application Number:
  US11/026,615
• Publication Number:
  US 20050221351A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00729 Peptide nucleic acids [PNA]
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 2201/06113 Coherent sources; lasers

Abstract

The present invention provides methods and devices for high sensitivity and high speed microarray optical imaging. In some embodiments, an electron multiplying CCD is used for microarray imaging.

Claims

1. A method for microarray imaging comprising:

Exciting a fluroscence labeled microarray with an excitation light source; and

Imaging the emission light pattern using an electron multiplying CCD.

2. The method of claim 1 wherein the microarray is a nucleic acid probe array.

3. The method of claim 2 wherein the microarray has at least 100,000 different probes per cm².

4. The method of claim 3 wherein the microarray is hybridized with targets that are labeled with a fluorescent label.

5. The method of claim 4 wherein the excitation light source is a uniformed excitation source.

6. The method of claim 5 wherein the excitation light source generates an excitation interference light pattern on the surface of the microarray.

7. The method of claim 6 wherein the exciting comprises generating a plurality of different excitation interference light pattern on the surface of the microarray.

8. The method of claim 7 wherein the imaging comprising obtaining a plurality of images.

9. The method of claim 8 wherein the exposure time for the images is less than 1000 msec.

10. The method of claim 9 wherein the exposure time is less than 500 msec.

11. The method of claim 1 wherein the microarray is a peptide probe array.

12. The method of claim 11 wherein the microarray has at least 100,000 different probes per cm².

13. The method of claim 12 wherein the microarray is bound with targets that are labeled with a fluorescent label.

14. The method of claim 13 wherein the excitation light source is a uniformed excitation source.

15. The method of claim 14 wherein the excitation light source generates an excitation interference light pattern on the surface of the microarray.

16. The method of claim 15 wherein the exciting comprises generating a plurality of different excitation interference light pattern on the surface of the microarray.

17. The method of claim 16 wherein the imaging comprising obtaining a plurality of images.

18. The method of claim 17 wherein the exposure time for the images is less than 1000 msec.

19. The method of claim 18 wherein the exposure time is less than 500 msec.