Method for purifying microbeads

• US 20050221360A1
• Filed: 03/25/2005
• Published: 10/06/2005
• Est. Priority Date: 03/31/2004
• Status: Abandoned Application

• Current Assignee: Takara Bio Incorporated (Ningbo Junsheng Investment Group Co., Ltd.)
• Original Assignee: Takara Bio Incorporated (Ningbo Junsheng Investment Group Co., Ltd.)
• Inventors: Asada, Kiyozo, Okamoto, Sachiko, Mineno, Junichi, Kato, Ikunoshin
• Application Number:
  US11/088,843
• Publication Number:
  US 20050221360A1
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: C12Q 1/6806 Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: C12Q 2525/161 incorporating target specific and non-target specific sites
• CPC Class Codes: C12Q 2563/107 fluorescence
• CPC Class Codes: C12Q 2563/125 the label being enzymatic, i.e. proteins, and non proteins, such as nucleic acid with enzymatic activity
• CPC Class Codes: C12Q 2565/102 Multiple non-interacting labels
• CPC Class Codes: C12Q 2565/1025 labels being on the same oligonucleotide
• CPC Class Codes: C12Q 2565/518 characterised by the immobilisation of the nucleic acid sample or target

Abstract

A method for purifying a microbead having an immobilized nucleic acid, a kit for the method, a microbead array which is an array of microbeads each having an immobilized nucleic acid, and a kit for preparing the microbead array.

Claims

1. A method for purifying a microbead having an immobilized nucleic acid as a target, the method comprising:

(a) immobilizing a nucleic acid labeled with at least two labeling substances to a microbead through a covalent bond, wherein at least one of the labeling substances is a detectable labeling substance and another labeling substance is a labeling substance capable of binding to a solid phase that is different from the microbead; and

(b) binding the microbead having the immobilized nucleic acid to the solid phase that is different from the microbead to isolate the bound microbead.

2. The method according to claim 1, wherein the at least two labeling substances are placed on the same molecule of the nucleic acid.

3. The method according to claim 1, wherein the detectable labeling substance is a labeling substance selected from the group consisting of fluorescent substances, chemiluminescent substances, enzymes and radioisotopes.

4. The method according to claim 1, wherein the labeling substance capable of binding to a solid phase that is different from the microbead is a labeling substance selected from the group consisting of biotin, avidin and haptens.

5. A kit for the method for purifying a microbead having an immobilized nucleic acid defined by claim 1.

6. A microbead array which is an array of microbeads each having an immobilized nucleic acid, wherein the nucleic acid as a target immobilized on each microbead consists of molecules having an identical sequence, the nucleic acid is labeled with at least two labeling substances, at least one of the labeling substances is a detectable labeling substance and another labeling substance is a labeling substance capable of binding to a solid phase that is different from the microbead.

7. The microbead array according to claim 6, wherein the at least two labeling substances are placed on the same molecule of the nucleic acid.

8. The microbead array according to claim 6, wherein the detectable labeling substance is a labeling substance selected from the group consisting of fluorescent substances, chemiluminescent substances, enzymes and radioisotopes.

9. The microbead array according to claim 6, wherein the labeling substance capable of binding to a solid phase that is different from the microbead is a labeling substance selected from the group consisting of biotin, avidin and haptens.

10. A kit for preparing the microbead array defined by claim 6.