Microfluidic devices for introducing and dispensing fluids from microfluidic systems
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Abstract
The present invention provides microfluidic devices, systems and methods for using the same, which facilitate the introduction of fluid to and from a microfluidic channel located within the microfluidic devices.
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Citations
66 Claims
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1-60. -60. (canceled)
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61. A method for producing a microfluidic fluid sampling device, said method comprising:
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producing a first elastomeric layer comprising a top surface, a bottom surface and an orifice;
producing a second elastomeric layer comprising a top surface, a bottom surface and a microscale recess disposed on the bottom surface;
attaching the bottom surface of the first elastomeric layer on to the top surface of the second elastomeric layer;
creating an orifice on the second elastomeric layer such that the orifice on the first elastomeric layer becomes operatively connected to and in fluid communication with the microscale recess located on the bottom surface of the second elastomeric layer; and
attaching a base layer to the bottom surface of the second elastomeric layer, thereby forming a microfluidic channel from the microscale recess, wherein the microfluidic channel is disposed within the interface of the second elastomeric layer and the base layer. - View Dependent Claims (62, 63, 64, 65, 66)
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Specification