Microarray based affinity purification and analysis device coupled with solid state nanopore electrodes
First Claim
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1. An apparatus for identifying a chemical moiety from a sample solution, comprising:
- (a) a substrate having a channel with at least one array for capturing and releasing a chemical moiety from a sample solution; and
(b) a solid state nanopore system downstream from the substrate for identifying the chemical moiety received from the substrate channel after the chemical moiety has been released from the array, the nanopore system comprising i. a first ring electrode, ii. a second ring adjacent to the first ring electrode;
iii. a nanopore adjacent to the first ring electrode and the second ring electrode and positioned to allow the chemical moiety to be positioned in the first ring electrode and the second ring electrode; and
iv. a voltage source for electrically connecting the first ring electrode to the second ring electrode for applying a ramping potential from the first ring electrode, through a portion of the chemical moiety in the nanopore to a second ring electrode to produce a signal indicative of the portion of the chemical moiety.
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Abstract
An apparatus and method for separating and identifying chemical moieties. The apparatus employs a microarray device coupled to a nanopore system. The apparatus both separates and identifies target molecules without the requirement of extraneous tags or fluorescent markers. Methods for using the apparatus are also disclosed.
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Citations
53 Claims
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1. An apparatus for identifying a chemical moiety from a sample solution, comprising:
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(a) a substrate having a channel with at least one array for capturing and releasing a chemical moiety from a sample solution; and
(b) a solid state nanopore system downstream from the substrate for identifying the chemical moiety received from the substrate channel after the chemical moiety has been released from the array, the nanopore system comprising i. a first ring electrode, ii. a second ring adjacent to the first ring electrode;
iii. a nanopore adjacent to the first ring electrode and the second ring electrode and positioned to allow the chemical moiety to be positioned in the first ring electrode and the second ring electrode; and
iv. a voltage source for electrically connecting the first ring electrode to the second ring electrode for applying a ramping potential from the first ring electrode, through a portion of the chemical moiety in the nanopore to a second ring electrode to produce a signal indicative of the portion of the chemical moiety. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. A method for separating and identifying a chemical moiety, comprising:
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(a) contacting a solution comprising a target molecule to a probe positioned in a channel of a substrate;
(b) capturing the target molecule from the sample by contacting the target molecule to the probe;
(c) releasing the target molecule from the probe; and
(d) identifying the target molecule translocating through the nanopore system by applying a ramping electrical current form a first ring electrode through a portion of the chemical moiety to a second ring electrode to identify a portion of the chemical moiety positioned in the nanopore.
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- 24. A method as recited in claim 24, further comprising translocating the chemical moiety through the nanopore to identify each of the translocating portions of the chemical moiety.
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30. An apparatus for identifying a chemical moiety from a sample solution, comprising:
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(a) a substrate having a channel with at least one array for capturing a chemical moiety from a sample solution; and
(b) a solid state nanopore system downstream from the substrate for identifying the chemical moiety received from the substrate channel after the chemical moiety has been released from the array, the nanopore system comprising i. a first electrode having a first nanopore, ii. a second electrode adjacent to the first electrode having a second nanopore wherein the first nanopore of the first electrode is positioned with the second nanopore of the second electrode so that the chemical moiety may translocate through the first nanopore and the second nanopore; and
iii. a voltage source for electrically connecting the first electrode to the second electrode for applying a ramping potential from the first electrode, through a portion of the chemical moiety in the nanopore to a second electrode to produce a signal indicative of the portion of the chemical moiety.
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- 33. An apparatus as recited in claim 33, further comprising a substrate for positioning 30 the first electrode and the second electrode.
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35. An apparatus for identifying a chemical moiety from a sample solution, comprising:
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(a) a substrate having a channel with at least one array for capturing a chemical moiety from a sample solution; and
(b) a solid state nanopore system downstream from the substrate for identifying the chemical moiety received from the substrate channel after the chemical moiety has been released from the array, the nanopore system comprising i. a first electrode, ii. a second electrode spaced from the first electrode to define a nanopore between the first electrode and the second electrode, the nanopore designed for receiving a tranlocating chemical moiety, the first electrode being in electrical connection with the second electrode; and
iii. a voltage source for electrically connecting the first electrode to the second electrode for applying a ramping potential from the first electrode, through a portion of the chemical moiety in the nanopore to a second electrode to produce a signal indicative of the portion of the chemical moiety.
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37. An apparatus for identifying a chemical moiety from a sample solution, comprising:
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(a) a substrate having a channel with at least one array for capturing a chemical moiety from a sample solution; and
(b) a solid state nanopore system downstream from the substrate for identifying the chemical moiety received from the substrate channel after the chemical moiety has been released from the array, the nanopore system comprising i. a first electrode layer having a first portion of the nanopore extending there through and exposing a first electrode edge;
ii. a first insulator layer adjacent to the first electrode layer, the first insulator layer having a second portion of the nanopore there through and defining a first insulator edge, the first insulator edge overhanging the first electrode edge;
iii. a second electrode layer adjacent to the first insulator layer, the second electrode layer having a third portion of the nanopore there through and defining a second electrode edge, the second electrode edge overhanging the first insulator edge;
wherein the first electrode and the second electrode may be electrically ramped for sensing the chemical moiety.
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- 40. A nanopore structure as recited in claim 40, further comprising a second insulator layer contacting the second electrode layer and being adjacent to the nanopore, the second insulator layer having a fourth portion of the nanopore there through and defining a second insulator edge, the second insulator edge overhanging the second electrode edge.
Specification