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Synthetic genes

  • US 20050227316A1
  • Filed: 04/07/2004
  • Published: 10/13/2005
  • Est. Priority Date: 04/07/2004
  • Status: Abandoned Application
First Claim
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1. A method for ligation of a plurality of DNA segments to obtain a ligation product that comprises sequences from each of said DNA segments in a predetermined order, said method comprising:

  • a) providing at least three DNA molecules, each comprising a DNA segment, wherein each segment is adjacent to one or two other segments in the ligation product, wherein each segment comprises a first region having sequence identity with a first adjacent DNA segment and a second region having sequence identity with a second adjacent DNA segment, if present;

    b) cleaving each DNA molecule to produce a DNA segment with one or two ligatable ends, each ligatable end comprising at least a portion of the region having sequence identity with an adjacent DNA segment;

    wherein at least one segment comprises two ligatable ends after cleavage, c) simultaneously ligating each segment to the adjacent segment or segments; and

    d) selecting a ligation product comprising sequences from each of said DNA segments in a predetermined order.

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