RNA interference mediated inhibition of TGF-beta and TGF-beta receptor gene expression using short interfering nucleic acid (siNA)
First Claim
1. A chemically synthesized double stranded short interfering nucleic acid (siNA) molecule that directs cleavage of a transforming growth factor beta receptor (TGF-betaR) RNA via RNA interference (RNAi), wherein:
- a) each strand of said siNA molecule is about 18 to about 23 nucleotides in length; and
b) one strand of said siNA molecule comprises nucleotide sequence having sufficient complementarity to said TGF-betaR RNA for the siNA molecule to direct cleavage of the TGF-betaR RNA via RNA interference.
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Abstract
This invention relates to compounds, compositions, and methods useful for modulating transforming growth factor beta (TGF-beta) and/or transforming growth factor beta receptor (TGF-betaR) gene expression using short interfering nucleic acid (siNA) molecules. This invention also relates to compounds, compositions, and methods useful for modulating the expression and activity of other genes involved in pathways of TGF-beta and/or TGF-betaR gene expression and/or activity by RNA interference (RNAi) using small nucleic acid molecules. In particular, the instant invention features small nucleic acid molecules, such as short interfering nucleic acid (siNA), short interfering RNA (siRNA), double-stranded RNA (dsRNA), micro-RNA (miRNA), and short hairpin RNA (shRNA) molecules and methods used to modulate the expression of TGF-beta and/or TGF-betaR genes.
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Citations
35 Claims
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1. A chemically synthesized double stranded short interfering nucleic acid (siNA) molecule that directs cleavage of a transforming growth factor beta receptor (TGF-betaR) RNA via RNA interference (RNAi), wherein:
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a) each strand of said siNA molecule is about 18 to about 23 nucleotides in length; and
b) one strand of said siNA molecule comprises nucleotide sequence having sufficient complementarity to said TGF-betaR RNA for the siNA molecule to direct cleavage of the TGF-betaR RNA via RNA interference. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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Specification